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当前位置: 首页 > 文章 > 耐低温螺旋藻新品系的诱变选育 安徽农业科学 2008,36 (29) 22-23
Position: Home > Articles > Mutant Screening of Low Temperature Tolerence Strain of Spirulina Journal of Anhui Agricultural Sciences 2008,36 (29) 22-23

耐低温螺旋藻新品系的诱变选育

作  者：
王妮;王素英;师德强
单  位：
天津市食品生物技术重点实验室;天津商业大学生物技术与食品科学学院
关键词：
钝顶螺旋藻;突变藻株;低温
摘  要：
[目的]筛选具有一定耐低温特性的螺旋藻新藻种。[方法]利用超声波处理钝顶螺旋藻,获得单细胞藻丝,用UV照射单细胞悬液,通过生长曲线测定,选出耐低温的突变藻株ZW1和ZW2,比较出发藻株和突变藻株的形态和生理生化特征。[结果]突变藻株的藻丝体变长,螺距加大,螺旋数目略有增加,最适生长温度低于出发藻株,而且在4℃低温处理6 h后,仍具有较高的放氧活性;ZW1藻体的蛋白质含量增幅为11.8%,ZW2的蛋白质和多糖含量的增幅分别为26.0%、28.6%。[结论]突变藻株ZW1、ZW2是耐低温藻种。
译  名：
Mutant Screening of Low Temperature Tolerence Strain of Spirulina
作  者：
WANG Ni et al(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134)
关键词：
Spirulina platensis;Mutants;Low temperature
摘  要：
[Objective] The research aimed to screen new stains of Spirulina with low temperature tolerance.[Method] Single cells of S.platensis were made by treating the initial strain with ultrasonic.Then these single cells were irradiated with UV laser.Through determining the growth rates-curve,the mutants ZW1 and ZW2 were selected.Their morphology,physiological features and biochemical composition were studied comparatively.[Result] The filaments of the mutants showed much longer lengh and spiral pitch than of the initial strain,the mutants could endure much lower temperature than the initial strain.And after treated for 6 h under 4 ℃,they still had higher oxygen evolving activity.The protein in ZW1 and ZW2 increased by 11.8%,26.0% respectively,and in ZW2 polysaccharide contents was 28.6% higher than that of their parent.[Conclusion] ZW1 and ZW2 were low temperature enduring strains.

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