中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 高蛋白野生大豆叶片氮代谢物及根瘤GSγ1和Glba表达分析 中国油料作物学报 2014,36 (3) 323-328
Position: Home > Articles > Comparison of leaf nitrogenous compounds and GSγ1/Glba expressions of nodule between Glycine max and G. soja with high protein content Chinese Journal of Oil Crop Sciences 2014,36 (3) 323-328

高蛋白野生大豆叶片氮代谢物及根瘤GSγ1和Glba表达分析

作  者：
韩红;张婷婷;王春红;汲添;于亭;杨美英
单  位：
吉林农业大学生命科学院
关键词：
高蛋白;野生大豆;栽培大豆;谷氨酰胺合成酶;血红蛋白;硝酸还原酶活性
摘  要：
为明确高蛋白野生大豆籽粒高蛋白形成过程中特有的遗传规律,以高蛋白栽培大豆为对照,对两类材料整个生育期叶片氮同化物相关指标,及根瘤谷氨酰胺合成酶基因(GSγ1)和豆血红蛋白基因(Glba)表达量的差异进行研究。结果表明:两类型大豆间存在明显差异。R3之后,栽培大豆比野生大豆叶片可溶性蛋白含量下降趋势明显。野生大豆叶片GSA在R3之前增长明显,R3之后下降缓慢。整个生育期间野生大豆叶片硝酸还原酶活性都要高于栽培大豆。野生大豆根瘤中GSγ1表达量在R3之前明显高于栽培大豆,R3期之后相反。从V6开始直到成熟,栽培大豆根瘤Glba的表达量都要高于野生大豆。说明野生大豆生育前期氮代谢及各种物质的合成、代谢较旺盛,而且能长时间地保持叶片高的可溶性蛋白含量及硝酸还原酶活性是籽粒高蛋白形成的原因之一。
译  名：
Comparison of leaf nitrogenous compounds and GSγ1/Glba expressions of nodule between Glycine max and G. soja with high protein content
作  者：
HAN Hong;ZHANG Ting-ting;WANG Chun-hong;JI Tian;YU Ting;YANG Mei-ying;College of Life Sciences,Jilin Agricultural University;
关键词：
High protein content;;Glycine soja;;G.max;;Glutamine syntheses;;Hemoglobin;;Nitrate reductase activity(NRA)
摘  要：
In order to clarify the formation mechanism of kernel high protein content in Glycine soja,the traits of leaf nitrogenous compounds and GSγ1,Glba expression of nodule in G. soja with high protein content were studied during the whole growth stage of soybean. G. max materials with high protein content were used as control. The results showed that there were significant differences between 2 types of soybean. The decreasing of leaf soluble protein content of G. max was sharper than that of G. soja after R3 stage. Glutamine syntheses activity( GSA) of G. soja increased obviously before R3 then decreased slowly after R3. Leaf nitrate reduction activity( NRA) of G. soja was higher than that of G. max during the entire growth period. The expression of GSγ1 in G. soja nodule was significantly higher than that before R3. However,the opposite result was obtained after R3. From V6 to maturity,the expression of Glba in G. max nodule was significantly higher than that in G. soja. All these indicated that the synthesis metabolism of nitrogenous compounds was very active,and the high content of soluble protein in leaf and the high activity of NRA were the other reasons for the formation of high kernel protein.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46