中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 基于RNA-Seq技术分析家蚕卵巢培养细胞(BmN)高表达基因 蚕业科学 2014,40 (3) 409-421
Position: Home > Articles > Analysis of Highly Expressed Genes in Bombyx mori Ovarian Culture Cells( BmN) Based on RNA-Seq Technology Science of Sericulture 2014,40 (3) 409-421

基于RNA-Seq技术分析家蚕卵巢培养细胞(BmN)高表达基因

作  者：
何蕾;薛仁宇;曹广力;胡小龙;贡成良
单  位：
苏州大学生命科学学院;苏州大学医学部基础医学与生物科学学院;苏州大学
关键词：
家蚕;BmN细胞;基因表达;GO注释;KEGG分析
摘  要：
为了更全面了解家蚕卵巢培养细胞(BmN)的转录基因及其涉及的功能,对BmN细胞进行深度RNA测序(RNAseq)。得到的总读段(reads)数为80 968 776条,有效RNA-Seq数据68 672 812条,reads的平均长度为94.85 bp,有效数据达到84.81%,证实测序数据可信。将有效数据比对家蚕mRNA序列,发现BmN细胞中的10 182个基因有不同程度表达。利用qRT-PCR测定随机选择BmN细胞中7个基因的相对表达水平,与RNA-Seq的结果略有差异,但总体趋势表现一致。以家蚕Actin3基因作为参照,计算BmN细胞中表达基因读段倍率变化的log2(Fold_change),将log2(Fold_change)>2的基因定义为高表达基因,共有41个基因在BmN细胞中高表达。选取FPKM值>15且表达水平最高的100个基因进行GO注释,主要涉及细胞组分、分子功能和生物学进程,富集在细胞组分中的基因比率高于富集在分子功能、生物学进程中的基因比率。KEGG分析显示这100个高表达基因分布在能量代谢,转录,翻译,折叠、组装和降解以及信号转导等22条通路上。比较发现BmN细胞与家蚕卵巢组织之间的高表达基因存在差异。
译  名：
Analysis of Highly Expressed Genes in Bombyx mori Ovarian Culture Cells( BmN) Based on RNA-Seq Technology
作  者：
HE Lei;XUE Ren-Yu;CAO Guang-Li;HU Xiao-Long;GONG Cheng-Liang;School of Biology and Basic Medical Sciences,Soochow University;National Engineering Laboratory for Modern Silk,Soochow University;
关键词：
Bombyx mori;;BmN cell;;Gene expression;;GO annotation;;KEGG analysis
摘  要：
To further understand the transcriptional genes and their functions of cultured BmN cells derived from silkworm(Bombyx mori) ovary,deep RNA sequencing of BmN cell was carried out. 80 968 776 paired-end reads were obtained based on RNA-Seq. After removing low-quality reads,68 672 812 paired-end reads with an average length of 94. 85 bp were obtained. The ratio of valid data to raw data was 84. 81%,confirming that the data were genuine and reliable. 10 182genes,which were expressed at various degrees in BmN cells,were obtained after blasting the valid data to mRNA sequences of silkworm. The qRT-PCR detection results of 7 randomly selected genes which were expressed in BmN cells were in line with high-throughput RNA sequencing results,also indicating that the RNA-Seq results in this study were credible. The log2(Fold_change) of genes relative to the constitutively expressed silkworm Actin3 gene were calculated,and the gene whose log2(Fold_change) was more than 2 was defined as highly expressed gene. The results showed that 41genes were highly expressed in BmN cells. GO analysis of the top 100 highly expressed genes with FPKM value >15indicated that these genes were mainly involved in cellular component,molecular function and biological process.The percentage of genes enriched in cellular component was higher than that in both molecular function and biological processes. KEGG analysis showed that these genes were involved in 22 pathways,mainly enriched in energymetabolism,transcription,translation,folding,sorting and degradation,and signal transduction. Moreover,expression differences of genes were found by comparing highly expressed genes in BmN cells with those in ovary.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46