中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 响应面法优化醇提荷叶总黄酮和生物碱的工艺研究 食品工业科技 2013,34 (18) 229-234，238
Position: Home > Articles > Study on the optimization of ethanol extraction of total flavonoids and alkaloids from lotus leaf using response surface methodology Science and Technology of Food Industry 2013,34 (18) 229-234，238

响应面法优化醇提荷叶总黄酮和生物碱的工艺研究

作  者：
霍丹群;冯丹;曾娜;代宇;周荣灵;侯长军
单  位：
重庆大学生物工程学院;国家固态酿造工程技术研究中心
关键词：
荷叶;总黄酮;总生物碱;响应面设计法
摘  要：
采用超声-酶-渗漉协同方法醇提荷叶总黄酮和生物碱,以总黄酮和生物碱的总评值为评价指标,用Box-Behnken响应面设计考察因素对其的影响,对结果进行二项式拟合及响应曲面分析得到最适宜提取条件为:乙醇浓度75%、料液比1∶26(g/mL)、超声时间80min、纤维素酶添加量14mg/g、渗漉速度3mL/min、渗漉液收集体积为9倍,在此条件下荷叶总黄酮和生物碱得率分别为6.7735%和0.7817%,与理论预测值偏差为-3%左右。实验结果表明,优化后的提取工艺稳定可行,且具有得率高、提取时间短等优点。
译  名：
Study on the optimization of ethanol extraction of total flavonoids and alkaloids from lotus leaf using response surface methodology
作  者：
HUO Dan-qun;FENG Dan;ZENG Na;DAI Yu;ZHOU Rong-ling;HOU Chang-jun;College of Bioengineering,Chongqing University;National Engineering Research Center of Solid-State Brewing Luzhou Laojiao Group Co.,Ltd.;
关键词：
lotus leaf;;total flavonoids;;alkaloids;;response surface method
摘  要：
The ethanol extraction process of lotus leaf total flavonoids and alkaloids was studied by ultrasonicenzyme-diacolation collaborative extraction technique.Box-Behnken response surface design method was used to investigate the effect of influence factors on overall desirability.The experimental data were analysed using second-order polynomial equation and 3D-response surface graphs.The optimum extraction conditions were as follows :ethanol concentration 75%,solid/liquid ratio 1∶26(g/mL),ultrasound time 80min,cellulase dosage 14mg/g,collecting 9 folds of percolate at a rate of 3mL/min,the proportion of total flavonoids and alkaloids was 6.7735% and 0.7817% under this condtions,respectively.The deviation between observed and predicted values of yield was-3%.The results indicated that the optimized extraction technique was a new method of higher efficiency and less time consumption.

相似文章

导出引用
计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46