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当前位置: 首页 > 文章 > 犬弓首蛔虫abcg-5基因的克隆及序列分析 西南大学学报(自然科学版) 2019 (3) 23-29
Position: Home > Articles > Cloning and Sequence Analysis of the Full-Length abcg-5 Gene of Toxocara canis Journal of Southwest University(Natural Science Edition) 2019 (3) 23-29

犬弓首蛔虫abcg-5基因的克隆及序列分析

作  者：
黄汉成;江艾耘;罗永莉;杨晓迪;李相兰;胡志刚;周荣琼
单  位：
西南大学动物科学学院
关键词：
犬弓首蛔虫;abcg-5基因;克隆;序列分析
摘  要：
研究犬弓首蛔虫(Toxocara canis,T.canis)腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporters, ABC)亚家族G5基因(abcg-5)的分子特征.根据犬弓首蛔虫基因组数据库中的Tc-abcg-5基因序列设计引物,通过PCR技术克隆Tc-abcg-5全长基因,并进行多重序列比对和种系发育进化树分析.结果表明:该基因全长为1 902 bp,编码633个氨基酸.功能结构域分析发现TcABCG5蛋白包含1个ABC转运蛋白结构域和6个跨膜区,同时发现了高度保守的Walker A和Walker B模体. GO分析显示ABCG5具有ATP结合和ATP酶活性.种系发育进化树分析显示TcABCG5与猪蛔虫进化关系较近,与哺乳动物进化关系较远.
译  名：
Cloning and Sequence Analysis of the Full-Length abcg-5 Gene of Toxocara canis
作  者：
HUANG Han-cheng;JIANG Ai-yun;LUO Yong-li;YANG Xiao-di;LI Xiang-lan;HU Zhi-gang;ZHOU Rong-qiong;College of Animal Science,Southwest University;
单  位：
HUANG Han-cheng%JIANG Ai-yun%LUO Yong-li%YANG Xiao-di%LI Xiang-lan%HU Zhi-gang%ZHOU Rong-qiong%College of Animal Science,Southwest University
关键词：
Toxocara canis;;ATP-binding cassette subfamily G member 5(abcg-5) gene;;cloning;;sequence analysis
摘  要：
To study the molecular characteristics of ATP-binding cassette sub-family G member 5(abcg-5) in Toxocara canis, the full-length coding sequence of Tc-abcg-5 gene was amplified using PCR amplification, multiple sequence alignment(MSA) was made and a phylogenetic tree was constructed. The results showed that the entire sequence of Tc-abcg-5 gene contained 1 902 bp, encoding a protein of 633 amino acids. TcABCG5 was found to contain one ABC transporter domain and six transmembrane regions. The highly conserved Walker A and Walker B motifs of the ABCG sub-family were also found. Gene ontology(GO) annotations showed that TcABCG5 had the molecular function of ATP binding and ATPase activity. Phylogenetic analysis indicated that TcABCG5 shared a high level of amino-acid sequence similarity with Ascaris suum, and shared a low level of amino-acid sequence similarity with mammals. This study is expected to lay a foundation for further functional researches of TcABCG5.

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