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Position: Home > Articles > PHYLOGENETIC ANALYSIS OF MITOCHONDRIAL 12S rRNA GENE OF TOXOCARA CANLS IN HENAN PROVINCE Chinese Journal of Animal Infectious Diseases 2017,25 (5) 64-67

基于12S rRNA基因序列的犬弓首蛔虫种系发育关系分析

作  者:
项黎丽;苏玉贤
单  位:
河南省济源市畜产品质量监测检验中心
关键词:
犬弓首蛔虫;线粒体DNA;12S rRNA基因;种系发育关系
摘  要:
本次研究旨在分析河南省不同地区犬弓首蛔虫分离株线粒体12S rRNA部分基因序列(plastiosome 12S rRNA,p12S rRNA)的遗传变异情况。通过PCR技术对犬弓首蛔虫p12S rRNA序列进行扩增并测序,应用Clustal X 2.0程序对序列进行比对,再利用Mega4.0程序进行NJ法绘制种系发育树。结果显示,所获得的18个犬弓首蛔虫分离株p12S rRNA序列长度为497~499 bp。种系发育分析结果显示,所有的犬弓首蛔虫分离株与已知犬弓首蛔虫位于同一分支,与其他蛔虫所属分支相隔较远。犬弓首蛔虫p12S rRNA序列种内相对保守,而种间差异较大,可以作为种间遗传变异研究的标记,从而为犬弓首蛔虫的分类与流行学调查奠定了基础。
译  名:
PHYLOGENETIC ANALYSIS OF MITOCHONDRIAL 12S rRNA GENE OF TOXOCARA CANLS IN HENAN PROVINCE
作  者:
XIANG Li-li;SU Yu-xian;Livestock Product Quality Surveillance Test Center of Jiyuan City,Henan Province;Animal Husbandry and Veterinary Station of Pingdingshan City Henan Drovince;
单  位:
Livestock Product Quality Surveillance Test Center of Jiyuan City,Henan Province%Animal Husbandry and Veterinary Station of Pingdingshan City Henan Drovince
关键词:
Toxocara canls;;mitochondrial DNA;;12S rRNA gene;;phylogenetic relationship
摘  要:
The aims of the present study were to analyze sequence variations in the mitochondrial 12S rRNA gene among Toxocara canls isolates from Henan province. The 12S rRNA was amplified from each of Toxocara canls isolates by PCR and analyzed using the Clustal X 2.0. The results showed that the lengths of all the 12S rRNA sequences were 497-499 bp. The 12 S r RNA sequences of the Henan isolates and reference Toxocara canls available in Gen Bank were aligned for phylogenetic analysis and found that they all clustered in the same clade. Therefore, 12S rRNA sequence could be used as a genetic marker for population genetic studies of ascarid. The results of the present study provided foundation for further studies of diagnosis and molecular epidemiology of Toxocara canls.

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