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当前位置: 首页 > 文章 > 螺旋藻富铬及其含铬蛋白组份对α-葡萄糖苷酶抑制的研究 安徽农业大学学报 2012,39 (4) 95-99
Position: Home > Articles > Effects of chromium(Ⅲ)-riched Spirulina platensis proteins on the activity inhibition of α-glucosidase Journal of Anhui Agricultural University 2012,39 (4) 95-99

螺旋藻富铬及其含铬蛋白组份对α-葡萄糖苷酶抑制的研究

作  者：
何颖敏;孙宜君;张娇;李博生
单  位：
北京林业大学螺旋藻研究所;北京林业大学生物科学与技术学院
关键词：
三价铬;螺旋藻蛋白;α-葡萄糖苷酶
摘  要：
在富铬Z氏培养液的条件下,研究了钝顶螺旋藻对Cr(Ⅲ)的吸收及其有机化程度,采用不同饱和度硫酸铵沉淀的方法分离提取不同蛋白组份,对其含Cr(Ⅲ)量进行了分析,并探讨了蛋白组份对α-葡萄糖苷酶活性的抑制作用。结果表明,螺旋藻培养液中CrCl3.6H2O添加浓度为300 mg·L-1,螺旋藻总铬富集量可达到3.30 mg·g-1,有机化程度为70.2%;不同饱和度(25%、50%和75%)硫酸铵沉淀出的蛋白组份中铬占总铬的情况分别为2.1%、16.8%和7.0%,不同蛋白质结合铬的能力各异;3种蛋白组份对α-葡萄糖苷酶均有抑制作用,其中50%饱和度硫酸铵沉淀的蛋白组份抑制活性最高,IC50为69.87 mg·mL-1。
译  名：
Effects of chromium(Ⅲ)-riched Spirulina platensis proteins on the activity inhibition of α-glucosidase
作  者：
HE Ying-min1,SUN Yi-jun 2,ZHANG Jiao3,LI Bo-sheng1(School of Biology Science and Technology,Beijing Forestry University,Beijing 100083)
关键词：
Chromium(Ⅲ);Spirulina platensis proteins;α-glucosidase
摘  要：
In this paper,we studied the absorption degree of Cr(Ⅲ) in Spirulina platensis which cultivated in chromium(Ⅲ)-riched Zarrouk medium,by extracting proteins with ammonium sulfate precipitation,analyzing the content of Cr(Ⅲ) in protein components.And then we investigated the inhibition effect of chromium(Ⅲ)-riched Spirulina platensis proteins on alpha-glucosidase.The results showed that Spirulina platensis growed in culture medium added with 300 mg?L-1 CrCl3?6H2O,the total enriched Cr(Ⅲ) and its organic degree of Cr(Ⅲ) could reach to 3.30 mg?g-1 and 70.2%,correspondingly.When different saturated solution(25%,50% and 75%) of ammonium sulfate were used to precipitate proteins,the proportion of Cr(Ⅲ) in protein components to total Cr(Ⅲ) were 2.1%,16.8% and 7.0%,respectively.The abilities of different proteins combined with Cr(Ⅲ) were different.The three protein components had different inhibition activities on alpha-glucosidase.Among them,protein com-ponents precipitated by 50% saturation solution of ammonium sulfate revealed the best inhibition effect on al-pha-glucosidase,with IC50 of 69.87 mg·mL-1.

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