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当前位置: 首页 > 文章 > 延边地区牛卵形巴贝斯虫病的分子流行病学调查 中国兽医杂志 2017 (8) 13-16
Position: Home > Articles > Molecular epidemiological servey of Babesia ovata infections in cattle in Yanbian Prefecture Chinese Journal of Veterinary Medicine 2017 (8) 13-16

延边地区牛卵形巴贝斯虫病的分子流行病学调查

作  者：
田万年;薛书江;贾立军;张守发;李国江
关键词：
卵形巴贝斯虫;PCR;血液涂片镜检法;分子流行病学调查
摘  要：
为了解牛卵形巴贝斯虫病在吉林省延边地区流行情况,本试验应用PCR方法对珲春地区190份牛的血液样本进行牛巴贝斯虫病的分子流行病学调查,并与血液涂片染色镜检法进行了比较。结果该地区牛巴贝斯虫病的PCR方法检测阳性率为30.52%,而血涂片染色镜检法的阳性率为11.05%。不同地区和不同饲养方式间牛卵形巴贝斯虫感染率差异显著(P<0.05)。遗传进化分析显示,吉林省延边地区牛卵形巴贝斯虫与日本分离株处于同一分支,而区别于河南和韩国分离株。调查表明,吉林省延边地区是牛卵形巴贝斯虫病的流行地区。
译  名：
Molecular epidemiological servey of Babesia ovata infections in cattle in Yanbian Prefecture
作  者：
TIAN Wan-nian;XUE Shu-jiang;JIA Li-jun;ZHANG Shou-fa;LI Guo-jiang;College of Animal Science, Jilin Agricultural Scienceand Technology College;Key Lab of Preventive Veterinary Medicine in Jilin Province;College of Agriculture, Yanbian University;
单  位：
College of Animal Science, Jilin Agricultural Scienceand Technology College%Key Lab of Preventive Veterinary Medicine in Jilin Province%College of Agriculture, Yanbian University
关键词：
Babesia ovata;;PCR;;microscopic examination of Giemsa-stained blood smears;;Molecular epidemiology
摘  要：
In order to study the prevalence ofBabesia ovatainfection in cattle in Yanbian area, a molecular epidemiological sur-vey was conducted to examineBabesia ovatausing PCR. 190 blood samples were analyzed. Results showed that the positive rate ofBabesia ovata infection by PCR method was 30. 52%, which was significantly higher than 11. 05% the positive rate by microscopicexamination of Giemsa-stained blood smears. The difference between different counties and grazing was both significant(p< 0. 05).Phylogenetic analysis indicated thatBabesia ovatain the present study was in the same cluster with the Japan strains, but was separa-ted from Henan or Korea strains. The survey confirmed that Yanbian Prefecture was the epidemic region ofBabesia ovata.

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