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Position: Home > Articles > 五株鸡滑液囊支原体的分离与鉴定 Hubei Agricultural Sciences 2018 (14)

五株鸡滑液囊支原体的分离与鉴定

作  者:
马爽;于国营;范根成;郭莉莉;赵鹏;宫晓;宋新宇
关键词:
鸡滑液囊支原体;分离;鉴定
摘  要:
从12份鸡滑液囊支原体临床病料中分离到5株支原体,经形态特性、生化特性、培养特性、分子生物学特性鉴定,5株支原体均为鸡滑液囊支原体.活菌计数结果表明,HN3株菌液的活菌数相对较高,为109CCU/m L;毒力试验结果表明,5株鸡滑液囊支原体对45日龄SPF鸡的发病率为50%~90%,HN3株毒力相对最高,为90%.
作  者:
MA Shuang;YU Guo-ying;FAN Gen-cheng;GUO Li-li;ZHAO Peng;GONG Xiao;SONG Xin-yu;Qingdao Yebio Bioengineering Co.,Ltd./State Key Laboratory of Animal Genetic Engineering Vaccine;
单  位:
MA Shuang%YU Guo-ying%FAN Gen-cheng%GUO Li-li%ZHAO Peng%GONG Xiao%SONG Xin-yu%Qingdao Yebio Bioengineering Co.,Ltd./State Key Laboratory of Animal Genetic Engineering Vaccine
关键词:
Mycoplasma synoviae;;isolation;;identification
摘  要:
Five strains of Mycoplasma synoviae were isolated from twelve suspected samples with clinical symptoms of Mycoplasma synoviae infection.Morphologic observation,biochemical test and molecular biological analysis were used to identify these strains. The results showed that the five strains were all Mycoplasma synoviae. Live bacteria counting results showed that Strain HN3 had highest number of living bacterium,109 CCU/m L. Virulence test results showed that morbidities of five strains to 45 days-old SPF chicken were 50%~90%. Strain HN3 had highest morbidity, was 90%.

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