中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鸡滑液囊支原体的RT-PCR检测及基因序列分析 贵州畜牧兽医 2019 (1) 45-47
Position: Home > Articles > RT-PCR Detection and Gene Sequence Analysis of Mycoplasma synoviae Guizhou Journal of Animal Husbandry & Veterinary Medicine 2019 (1) 45-47

鸡滑液囊支原体的RT-PCR检测及基因序列分析

作  者：
田浪;温贵兰;程振涛;王开功;文明;周碧君;张升波;杨佰启;李昌红;徐丽;张喜懿;陈广
单  位：
贵州大学动物科学学院预防兽医实验室;贵州省动物生物制品工程技术研究中心
关键词：
鸡滑液囊支原体;RT-PCR检测;基因序列分析;鉴别诊断
摘  要：
为确诊某鸡场肉鸡发病死亡的病因,通过临床症状、病理变化、RT-PCR检测、病原S1基因序列分析进行综合诊断。结果:(1)病鸡表现食欲不振、精神沉郁、跛行及无法站立、关节肿大等症状,剖检发现肿大关节内有大量黏液。(2)鸡滑液囊支原体RT-PCR扩增出现207 bp特异性条带,与预期结果一致。(3)病原S1基因序列分析显示与GenBank公布的鸡滑液囊支原体基因序列同源性均达到99%,构建系统进化树分析显示与GenBank公布的参考株聚为1支。综合诊断为鸡滑液囊支原体感染。
译  名：
RT-PCR Detection and Gene Sequence Analysis of Mycoplasma synoviae
作  者：
Tian Lang;Wen Guilan;Cheng Zhentao;Wang Kaigong;Wen Ming;Zhou Bijun;Zhang Shengbo;Yang Baiqi;Li Changhong;Xu Li;Zhang Xiyi;Chen Guang;Laboratory of Preventive Veterinary Medicine,Institute of Animal Science,Guizhou University;Animal Biological Engineering Technology Research Center in Guizhou Province;
单  位：
Tian Lang%Wen Guilan%Cheng Zhentao%Wang Kaigong%Wen Ming%Zhou Bijun%Zhang Shengbo%Yang Baiqi%Li Changhong%Xu Li%Zhang Xiyi%Chen Guang%Laboratory of Preventive Veterinary Medicine,Institute of Animal Science,Guizhou University%Animal Biological Engineering Technology Research Center in Guizhou Province
关键词：
Mycoplasma synoviae;;RT-PCR Detection;;Gene Sequence Analysis;;Differential Diagnosis
摘  要：
In order to identify the cause of death in a chicken farm,the comprehensive diagnosis was made by clinical symptoms,pathological changes,RT-PCR detection,and pathogen S1 gene sequence analysis. Result:( 1) The sick chicken showed symptoms such as loss of appetite,depression,lameness and inability to stand,joint swelling,etc.,necropsy found a large amount of mucus in the enlarged joint.( 2) The 207 bp specific band appeared in the RT-PCR of Mycoplasma synoviae,which was consistent with the expected results.( 3) Sequence analysis of the pathogen S1 gene showed that the sequence homology with the Mycoplasma synoviae gene published by Gen Bank reached 99%. The phylogenetic tree analysis showed that the reference strain published by GenBank was clustered into one. The diagnosis was Mycoplasma synoviae infection.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46