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Position: Home > Articles > Regulatory mechanism of DOPA decarboxylase (DDC) on fecundity in Harmonia axyridis (Coleoptera: Coccinellidae) Acta Entomologica Sinica 2022,65 (1) 1-9

多巴脱羧酶对异色瓢虫生殖力的调控机制

作  者：
陈旭;田仁斌;徐庆宣;李姝;王甦;臧连生;肖达
单  位：
北京市农林科学院植物保护环境保护研究所;贵州大学绿色农药与农业生物工程教育部重点实验室
关键词：
异色瓢虫;多巴胺;多巴脱羧酶;RNAi;生殖力
摘  要：
【目的】为了探究多巴脱羧酶(DOPA decarboxylase, DDC)对异色瓢虫Harmonia axyridis生殖力的影响及其调控机制。【方法】利用RNA干扰技术(RNA interference, RNAi),将异色瓢虫4龄幼虫的DDC基因(HaDDC)抑制表达,成虫羽化后第8天开始统计20 d内累计产卵量及产卵后第3天统计子代卵孵化率。同时解剖异色瓢虫雌成虫卵巢,观察比较处理组(dsHaDDC注射组)和对照组(dsGFP注射组)羽化后第8天成虫卵巢组织形态并统计卵巢管数量;观察比较处理组和对照组羽化后第14和20天成虫卵巢管中卵的发育情况。【结果】异色瓢虫雌雄成虫HaDDC基因均通过RNAi被抑制表达后,成虫20 d内累计产卵量为38.67±7.80粒,与对照组(371.33±84.31粒)相比显著降低;雌成虫或雄成虫HaDDC基因被抑制表达后,20 d内累计产卵量分别为135.50±28.38和76.00±14.00粒,均与对照组差异显著。HaDDC基因均被抑制表达的雌雄成虫交配后,其子代卵孵化率为0。异色瓢虫成虫羽化后第8天,处理组中雌虫的卵巢组织形态和卵巢管数量与对照组无显著差异。羽化后第14天的异色瓢虫成虫卵巢形态饱满,双侧卵巢管中有卵母细胞发育,但与对照组相比,处理组卵巢组织中未见成熟的卵粒。【结论】基于上述研究结果,初步判断多巴脱羧酶通过参与异色瓢虫卵母细胞到成熟卵粒的发育过程,进而调控其生殖力。
译  名：
Regulatory mechanism of DOPA decarboxylase (DDC) on fecundity in Harmonia axyridis (Coleoptera: Coccinellidae)
作  者：
CHEN Xu;TIAN Ren-Bin;XU Qing-Xuan;LI Shu;WANG Su;ZANG Lian-Sheng;XIAO Da;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences;Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University;
关键词：
Harmonia axyridis;;dopamine;;DOPA decarboxylase;;RNAi;;fecundity
摘  要：
【Aim】The objective of this research is to explore the effects of DOPA decarboxylase(DDC) on fecundity in the multicolored Asian beetle, Harmonia axyridis, and its regulatory mechanism. 【Methods】RNA interference(RNAi) was used to suppress the expression of DDC gene(HaDDC) in the 4 th instar larvae of H. axyridis, and the cumulative number of eggs laid in 20 d was counted from the 8 th day after adult emergence and the egg hatching rate of offspring was recorded on the 3 rd day after egg laying. Then the ovaries of female adults of H. axyridis were dissected, the ovarian tissue morphology and the number of ovarioles of the 8-day-old adults between the treatment group(injected with dsHaDDC) and the control group(injected with dsGFP), and the development of eggs in the ovarioles of the 14-and 20-day-old adults between the treatment group and the control group were observed and recorded.【Results】After the expression of HaDDC gene of both sexes of H. axyridis was inhibited by RNAi, the cumulative number of eggs laid by adults in 20 d was 38.67±7.80, which was significantly lower than that of the control group(371.33±84.31). After the expression of HaDDC gene in female and male adults was inhibited, the cumulative numbers of eggs laid in 20 d were 135.50±28.38 and 76.00±14.00, respectively, which were significantly different from that of the control group. The egg hatching rate of offspring was 0 when the expression of HaDDC gene in both sexes was inhibited. On the 8 th day after emergence of H. axyridis, the ovarian tissue morphology and the number of ovarioles in the treatment group were not significantly different from the control. On the 14 th day after adult emergence, the ovaries of H. axyridis were plump, and the oocytes were developed in the bilateral ovarioles. However, compared with the control group, no mature eggs were found in the treatment group.【Conclusion】Based on the above results, we can preliminarily conclude that DDC can regulate the fecundity of H. axyridis by participating in the development process from oocyte to mature eggs.

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