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当前位置: 首页 > 文章 > 不同色型异色瓢虫转录组差异表达基因分析 植物保护学报 2021 (6) 1341-1350
Position: Home > Articles > Analyses of differentially expressed genes in transcriptomes of multicolored Asian lady beetle Harmonia axyridis with different color patterns Journal of Plant Protection 2021 (6) 1341-1350

不同色型异色瓢虫转录组差异表达基因分析

作  者：
陈旭;陈梦瑶;王甦;臧连生;肖达
单  位：
北京市农林科学院植物保护环境保护研究所;浙江大学昆虫科学研究所浙江省作物病虫生物学重点实验室;贵州大学绿色农药与农业生物工程教育部重点实验室
关键词：
异色瓢虫;色型多样性;转录组;能量代谢;氧化磷酸化
摘  要：
为探究不同色型异色瓢虫Harmonia axyridis之间产生生理学差异的机制,通过Illumina HiSeq测序平台对黄底多窗型、黑底两窗型和黑底四窗型3种不同色型异色瓢虫雌雄成虫的18个转录组进行高通量测序,比较不同色型间的差异表达基因,并结合GO和KEGG数据库对差异表达基因进行注释分析。结果表明:通过对差异表达基因进行定量比较发现,与黄底多窗型相比,2种黑底型异色瓢虫雌雄成虫的下调基因明显更多;GO功能分类结果显示,不同色型的异色瓢虫雄成虫和雌成虫差异表达基因所属分类基本相似,并未见显著富集现象,且主要属于细胞过程、单组织活动和细胞等类别;通过KEGG数据库比对分析发现不同色型异色瓢虫中的差异表达基因功能主要与环境适应和能量代谢通路相关。其中,与氧化磷酸化过程相关的基因在2种黑底型异色瓢虫中的表达量显著低于黄底多窗型异色瓢虫,这种差异在雌成虫中更为显著。
译  名：
Analyses of differentially expressed genes in transcriptomes of multicolored Asian lady beetle Harmonia axyridis with different color patterns
作  者：
Chen Xu;Chen Mengyao;Wang Su;Zang Liansheng;Xiao Da;Key Laboratory of Green Pesticide and Agricultural Bioengineering of Ministry of Education, Guizhou University;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences;Key Laboratory of Crop Pest Biology of Zhejiang Province, Institute of Insect Sciences of Zhejiang University;
关键词：
Harmonia axyridis;;phenotypic diversity;;transcriptome;;energy metabolism;;oxidative phosphorylation
摘  要：
In order to explore the mechanism of physiological differences among different color patterns of multicolored Asian lady beetle Harmonia axyridis, 18 transcriptomes of three color(yellow, blackwith-two-plot and black-with-four-plot) patterns were sequenced by Illumina HiSeq platform. The differentially expressed genes between different color patterns were compared and annotated against GO and KEGG databases. The results showed that two black patterns had higher number of down-regulated genes than the yellow pattern. The results of GO functional classification showed that the differentially expressed genes of male and female ladybirds with different color patterns were basically similar, and there was no significant enrichment, which were mainly related with cell processes, single tissue activities and cells. Through comparison and analysis against the KEGG database, the differentially expressed genes of different color patterns of H. axyridis were mainly related to environmental adaption and energy metabolism. Among them, the number of genes related to oxidative phosphorylation in two black patterns were significantly lower than that of the yellow pattern, with a more significant difference in female.

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