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当前位置: 首页 > 文章 > 多环芳烃分子印迹柱-高效液相色谱荧光检测法快速测定烤肉中15种多环芳烃 肉类研究 2018 (11) 47-52
Position: Home > Articles > Determination of 15 Polycyclic Aromatic Hydrocarbons in Barbecued Meat by Molecularly Imprinted Solid-Phase Extraction Combined with High Performance Liquid Chromatography-Fluorescence Detection MEAT RESEARCH 2018 (11) 47-52

多环芳烃分子印迹柱-高效液相色谱荧光检测法快速测定烤肉中15种多环芳烃

作  者：
阳文武;谭顺中;郭娅;周浓
单  位：
重庆三峡学院生物与食品工程学院;重庆市万州食品药品检验所
关键词：
多环芳烃;烤肉;高效液相色谱-荧光检测;多环芳烃分子印迹柱;外标法定量
摘  要：
建立一种高效液相色谱-荧光检测(high performance liquid chromatography-fluorescence detection,HPLC-FLD)法同时测定烤肉中15种多环芳烃(polycyclic aromatic hydrocarbons,PAHs)的方法。样品经超声提取,多环芳烃分子印迹柱(molecular imprinting column of PAHs,MIP-PAHs)净化,通过HPLC分离,外标法定量。结果表明:15种PAHs在质量浓度1～50 ng/mL范围内与色谱峰面积呈良好的线性关系,r>0.999 5;在5.0、10.0、25.0μg/kg 3个加标水平下,平均回收率为71.1%～98.8%,相对标准偏差为1.0%～5.8%;方法检出限(R_(S/N)=3)为0.33～3.30μg/kg,定量限(R_(S/N)=10)为1.0～10.0μg/kg。
译  名：
Determination of 15 Polycyclic Aromatic Hydrocarbons in Barbecued Meat by Molecularly Imprinted Solid-Phase Extraction Combined with High Performance Liquid Chromatography-Fluorescence Detection
作  者：
YANG Wenwu;TAN Shunzhong;GUO Ya;ZHOU Nong;Chongqing Wanzhou Institute for Food and Drug Control;College of Biology and Food Engineering, Chongqing Three Gorges University;
单  位：
YANG Wenwu%TAN Shunzhong%GUO Ya%ZHOU Nong%Chongqing Wanzhou Institute for Food and Drug Control%College of Biology and Food Engineering, Chongqing Three Gorges University
关键词：
polycyclic aromatic hydrocarbons;;barbecued meat;;high performance liquid chromatography-fluorescence detection;;molecular imprinting column of PAHs;;external standard quantitation
摘  要：
A method for simultaneous determination of 15 polycyclic aromatic hydrocarbons(PAHs) in barbecued meat was developed by high performance liquid chromatography with fluorescence detection(HPLC-FLD). Samples were ultrasonically extracted, and the extract was purified by molecularly imprinted solid-phase extraction(MISPE), separated by HPLC and quantified by external standard method. The results showed that the concentration of 15 PAHs in the range from 1 to 50 ng/m L was linear with the chromatographic peak area with correlation coefficients(r) more than 0.999 5. Average recoveries at spiking levels of 5.0, 10.0 and 25.0 μg/kg ranged from 71.1% to 98.8% with relative standard deviations(RSDs) from 1.0% to 5.8%. The detection limits(R_(S/N) = 3) varied from 0.33 to 3.30 μg/kg and the quantitation limits(R_(S/N) = 10) from 1.0 to 10.0 μg/kg.

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