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胶体金试纸条快速检测食品中磺胺二甲基嘧啶残留

作  者:
林晓丽;饶辉;熊勇华;陈媛;刘文娟;赖卫华
单  位:
江西中德生物工程有限公司;江西省兽药饲料监察所;南昌大学
关键词:
磺胺二甲基嘧啶;胶体金;试纸条;快速检测
摘  要:
目的:旨在建立一种快速、简便的针对磺胺二甲基嘧啶残留的检测方法。方法:应用竞争抑制免疫层析原理,研制磺胺二甲基嘧啶胶体金试纸条。结果:该试纸条检测限为10ng/mL、检测时间5min,与其他磺胺类药物交叉反应小,可用于多种食品样本的检测,且样本检测限为100μg/kg。结论:该方法操作简便、灵敏度高且特异性强,可实现对食品样品中磺胺二甲基嘧啶残留的快速检测。
译  名:
Rapid Detection of Sulfadimidine Residue in Food by Colloidal Gold Strips
作  者:
LIN Xiao-li1,Rao Hui 2,XIONG Yong-hua1,CHEN Yuan3,LIU Wen-juan3,LAI Wei-hua1, (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. Jiangxi Provincial Institute of Veterinary Drug and Feed Control, Nanchang 330029, China; 3. Jiangxi Zodolabs Biological Engineering Co. Ltd., Nanchang 330029, China )
关键词:
sulfadimidine;colloidal gold;strip;rapid detection
摘  要:
Objective: To establish a rapid and convenient detection method for sulfadimidine residue in food. Methods: The colloidal gold strip for specific sulfadimidine was developed successfully on the basis of competitive inhibition immunochromatography mechanism. Results: The detection limit of the colloidal gold strip was 10 ng/mL for sulfadimidine within less than 5 min, which also had low cross-reactivity with other sulfonamides. The colloidal gold strip could be used in rapid detection of sulfadimidine residue in many kinds of food samples with detection limit of 100 μg/kg. Conclusion: This method is characteristics of simplicity, high sensitivity and specificity to achieve the rapid detection of sulfadimidine residue in food.

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