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当前位置: 首页 > 文章 > 内生真菌ZPRa-R-1对红景天中关键信号分子及主要次生代谢物的影响 植物研究 2016,36 (3) 416-420
Position: Home > Articles > Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata Bulletin of Botanical Research 2016,36 (3) 416-420

内生真菌ZPRa-R-1对红景天中关键信号分子及主要次生代谢物的影响

作  者：
王梦亮;焦晋;邢婕;田俊生;崔晋龙
单  位：
首都师范大学生命科学学院;山西大学应用化学研究所;山西大学应用化学研究所山西大学生物技术研究所山西大学中医药现代研究中心
关键词：
红景天;内生真菌;信号分子;酪醇;红景天苷
摘  要：
研究内生真菌ZPRa-R~(-1)对大花红景天组培苗中信号分子NO,SA和H_2O_2,及主要次生代谢物酪醇(p-tyrosol)和红景天苷(salidroside)积累的影响。分别采用Greiss试剂法、RP-HPLC荧光法、硫酸钛法检测NO,SA和H_2O_2等信号分子;采用分光光度法检测关键酶PAL和CA4H的活性;采用RP-HPLC检测酪醇和红景天苷。ZPRa-R~(-1)接种于红景天,首先引起宿主信号分子NO含量增高,其次是SA和H_2O_2,它们达到最大值的时间分别是共生后第8,10和12 d,含量分别为0.193μmol·g~(-1),0.062μg·g~(-1),0.092μmol·g~(-1);关键酶PAL和CA4H活性最大值分别为166.400 U·g~(-1)和0.625 U·g~(-1)·h~(-1),分别是对照组的8和1.5倍,最终引起酪醇和红景天苷的积累效应,在第8和10 d达到最大值,含量分别为8.656和0.498 mg·g~(-1),是对照组的7和2.8倍。研究结果表明内生真菌ZPRa-R~(-1)能通过诱导激活宿主信号分子网络系统,引起关键酶活性的变化,进而调控酪醇和红景天苷等次生代谢物的积累。
译  名：
Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata
作  者：
WANG Meng-Liang;JIAO Jin;XING Jie;TIAN Jun-Sheng;CUI Jin-Long;WANG Jun-Hong;Insititute of Applied Chemistry,Shanxi University;Insititute of Biotechnology,Shanxi University;Modern Research Center for Traditional Chinese Medicine,Shanxi University;
关键词：
Rhodiola crenulata;;endophytic fungi;;signal molecule;;p-tyrosol;;salidroside
摘  要：
We studied the effects on the key signal molecules and the main secondary metabolites' s content in tissue culture of Rhodiola crenulata inoculated with endophic fungi ZPRa-R~(-1). We selected Greiss method,RPHPLC and Titanium sulfate method to detect the contents of NO,SA and H_2O_2,respectively,and detected the key enzymes,PAL and CA4 H by spectrophotometry. p-Tyrosol and salidroside in Rhodiola seedling co-cultured with fungi were evaluated by RP-HPLC. The content of NO increased firstly,followed by SA and H_2O_2 after that Rhodiola seedling inoculated with endophic fungi. The maximum contents of NO,SA and H_2O_2 were detected at8,10 and 12 d,and they were 0. 193 μmol·g- 1,0. 062 μg·g- 1and 0. 092 μmol·g- 1,respectively. The maximum contents of PAL and CA4 H were 166. 400 U·g- 1,0. 625 U·g- 1·h- 1,and were 8 and 1. 5 times as much as that of control,repectively. The contents of p-tyrosol and salidroside were accumulated,and the time of each of them reaching the maximum content was on 8 and 10 d,and the contents were 8. 656 and 0. 498mg·g- 1,and were 7 and 2. 5 times as much as that of control,respectively. Therefore,ZPRa-R~(-1) has the capacity of regulating signals and promoting the activities of PAL and CA4 H,and finally promoted accumulation of p-tyrosol and salidroside in co-culturing seedling of fungi and Rhodiola.

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