当前位置: 首页 > 文章 > 一株乌天麻共生萌发菌培养条件的优化 北方园艺 2016 (19) 175-177
Position: Home > Articles > Optimization of Cultivation Condition in Gastrodia elata Seed-germinating Strain Northern Horticulture 2016 (19) 175-177

一株乌天麻共生萌发菌培养条件的优化

作  者:
程立君;李世辉;胡志芳;段相程;赵艳娟
单  位:
昭通苹果产业研究所;昭通学院化学与生命科学学院
关键词:
天麻;萌发菌;环境条件;营养条件
摘  要:
以天麻共生萌发菌MY-001为试材,采用单因素试验和正交实验分析法,以菌丝生长速率和菌丝体生物量为主要指标,对萌发菌MY-001培养环境条件及培养基营养条件进行优化。结果表明:MY-001最适宜的环境条件为温度25℃,培养基质含水量200%下进行暗培养;MY-001最易利用的氮源和碳源分别是酵母膏、葡萄糖,复合维生素B也能促进菌丝生长,最佳的营养条件为酵母膏3g·L~(-1)、葡萄糖20g·L~(-1)、复合维生素B 0.005g·L~(-1),通过多元回归分析建立回归方程(Y=0.362+0.084A-0.014B-0.007C),方差分析显示方程为极显著。
译  名:
Optimization of Cultivation Condition in Gastrodia elata Seed-germinating Strain
作  者:
CHENG Lijun;LI Shihui;HU Zhifang;DUAN Xiangcheng;ZHAO Yanjuan;College of Chemistry and Life Sciences,Zhaotong University,Zhaotong Characteristic Agricultural Products Research Center;Zhaotong Apple Industry Research Institute;
关键词:
Gastrodia elata;;germinating strain;;environmental condition;;nutrient condition
摘  要:
Gastrodia elataseed-germinating strain MY-001 was used as test material,the cultivation conditions of MY-001 was studied according to the index of mycelial growth rate and mycelial biomass by single factor experiment and orthogonal experiment.The results showed that the optimum growth environmental conditions were 25℃,200% water content,under dark cultivation;glucose and yeast extracts were the optional carbon source and nitrogen sources.The optimum growth nutrient conditions were determined yeast extracts 3g·L~(-1),glucose 20g·L~(-1),composite vitamin B0.005g·L~(-1).Through multiple regression analysis to establish regression equation(Y=0.362+0.084A-0.014B-0.007C),variance analysis showed significant equation was very significant.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊