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当前位置: 首页 > 文章 > 茶树花活性成分的分析与鉴定 食品科学 2007,28 (7) 400-403
Position: Home > Articles > Identification and Analysis of Major Bioactive Compounds in Tea Flower FOOD SCIENCE 2007,28 (7) 400-403

茶树花活性成分的分析与鉴定

作  者：
黄阿根;董瑞建;韦红
单  位：
扬州大学食品科学与工程学院
关键词：
茶树花;多酚;多糖;气相色谱法;高效液相色谱法
摘  要：
茶树花是茶叶生长过程中的废弃物,很少加以利用。为了分析和鉴定出茶树花有效成分,便于其开发利用,选取了5个地方的茶树花品种,对其进行成分分析。采用分光光度法测定多酚、总糖和多糖的含量。用酒石酸亚铁比色法进行测定多酚,用苯酚-硫酸法测定茶树花总糖和多糖。茶树花多糖用岛津GC-14B型气相色谱仪分析其组成,茶树花多酚用美国Thermo公司SpectraSystem型高效液相色谱仪分析其组成。对鲜茶树花不同部位主要化学成分进行了比较。实验表明:①茶树花中含多酚6.35%～8.19%,茶树花多酚由表没食子儿茶素(EGC)、表没食子儿茶素没食子酸酯(EGCG)、表儿茶素(EC)、没食子儿茶素没食子酸酯(GCG)、表儿茶素没食子酸酯(ECG)儿茶素类化合物组成。②茶树花总糖含量为20.97%～33.60%,多糖含量为1.04%～1.84%。茶树花多糖单体有葡萄糖、阿拉伯糖、半乳糖、半乳糖醛酸、甘露糖、木糖、岩藻糖、鼠李糖等。③花蕊所含的活性成分稍高于花瓣。分析结果表明茶树花含多酚和多糖,并可以开发利用作为营养保健品。
译  名：
Identification and Analysis of Major Bioactive Compounds in Tea Flower
作  者：
HUANG A-gen,DONG Rui-jian,WEI Hong (College of Food Science and Technology, Yangzhou University, Yangzhou 225001,China)
关键词：
tea flower;polyphenols;polysaccharidies;GC;HPLC
摘  要：
Tea flower [Camellia sinensis (L.) O.kuntze] is trimmed by growers in order to increase the production and improve the quality of the tea leaves. However the flower is rarely utilized for further applications. Tea flower samples from five species of five locations were selected and analyzed for the identification and utilization of the bioactive components. The contents of polypheols, total sugars, and polysaccharides were respectively determined by spectrophotometric methods. The polysaccharide components were isolated with gas chromatography(GC-14B, SHIMADZU, Japan), and the polyphenol components were identified with high pertormance liquid chromatography (HPLC-Spectra System, THERMO, America) with C18 column (0.46 mm×150 mm). The results showed that camellia samples contain high levels of total saccharides, polyphenols and soluble sugars, at 20.97%～33.60%, 6.35%～ 8.19%, and 1.04%～1.84%, respectively. The polyphenols are comprised of epigallocatechin, epigallocatechin gallate, epicatechin, gallocatechin gallate and epicatechin gallate. The polysaccharides are composed of glucose, arabinose, galactose, galacturonic acid, mannose, xylose, fucose and rhamnose. These samples have a similarity in major bioactive compounds to the tea leaves, even though their average content of polysaccharides is significantly higher than that of the tealeaves. The distribution of these components in camellia was also examined. The bioactive materials are higher in pistil than in petal. Overall, the tea flower samples have higher levels of bioactive components than tea leaves and are worthy further exploitation for nutraceuticals.

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