MICROPROPAGATION SYSTEM AND GERMPLASM PESERVATION IN VITRO OF VACCINIUM ULIGINOSUML.VAR.ALPINUM NAKAI.

GU Di-zhou,GU Mei-ying,CAO Xun,JIANG Yun-tian,ZHU Jun-yi (Department of Biology,Tonghua Normal University,Tonghua134002,China)

Vaccinium uliginosum L.var.alpinum Nakai.;Micropropagation;peservation in vitro;uniform design;phloridzin

The tender buds of Vaccinium uliginosum L.var.alpinum Nakai.were used as explants for the experiment.Uniform design for the most suitable media for shoot regeneration immediately at base of tender buds,rooting and germplasm preservation in vitro was screened.The results showed that DR+TDZ2.0 mg·L-1+IBA 0.01 mg·L-1 was fits for shoot regeneration,the rate of regeneration was more than 98.8%;MS(modified)+IAA0.5 mg·L-1+IBA0.07 mg·L-1+Kt0.1 mg·L-1for rooting,the rate of rooting was 98%;1/5MS+B91.8 mg·L-1+phloridzin3.3 mg·L-1for germplasm preservation in vitro for 43 months.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a 60-fold proliferation rate was achieved within 35 days.The method of "low nutrients with defering growth and dwarfing" was utilized for germplasm peservation in vitro at normal temperature.In vitro culture and germplasm peservation in vitro system of Vaccinium uliginosum L.var.alpinum Nakai.has been successfully established.