当前位置: 首页 > 文章 > 新疆家蚕抗菌肽可有效抑制掌面革兰氏阴性菌的研究 中国畜牧兽医 2014,41 (9) 247-252
Position: Home > Articles > Research on CecropinXJ Effectively Inhibited the Palm Gram-negative Bacteria China Animal Husbandry & Veterinary Medicine 2014,41 (9) 247-252

新疆家蚕抗菌肽可有效抑制掌面革兰氏阴性菌的研究

作  者:
康苏;夏丽洁;刘军;马纪;张富春
单  位:
新疆大学生命科学与技术学院;新疆生物资源基因工程重点实验室
关键词:
新疆家蚕抗菌肽;手掌表面细菌;平板菌落计数;革兰氏阴性菌
摘  要:
抗菌肽具有抑制细菌的作用,是天然防腐剂和潜在的抗生素替代品。为了解新疆家蚕抗菌肽(CecropinXJ)能否有效抑制手掌表面细菌,本研究采用擦拭法获取手掌表面细菌擦拭液,并用不同浓度CecropinXJ处理手掌擦拭液,通过平板菌落计数法评价CecropinXJ的抑菌效果,结果表明随着CecropinXJ浓度增加,其抑菌能力越好。当CecropinXJ添加到0.4%时,抑菌能力达到商业洗手液水平(P>0.05);进一步对16SrDNA进行测序分析,结果发现手掌表面细菌多为革兰氏阴性菌,添加CecropinXJ后,手掌表面的革兰氏阴性菌生长能有效被抑制。本试验结果表明CecropinXJ可以用作消毒清洁产品进行开发。
译  名:
Research on CecropinXJ Effectively Inhibited the Palm Gram-negative Bacteria
作  者:
KANG Su;XIA Li-jie;LIU Jun;MA Ji;ZHANG Fu-chun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;
关键词:
CecropinXJ;;palm surface bacteria;;plate count;;gram-negative bacteria
摘  要:
Antimicrobial peptide(AMP)could kill bacteria,which had been used as a natural preservative and potential alternative to antibiotics.To understand whether the CecropinXJ could effectively inhibit bacteria from palm skin,we used sterile water to swab hands and treated with swabbed liquor by different concentrations of CecropinXJ in this study.The antimicrobial activity was evaluated by plate count method.The results showed that the growth of bacteria derived from palm skin could be effectively inhibited via adding different concentrations of CecropinXJ,and the antimicrobial activity became stronger with increasing the concentrations of CecropinXJ.When the concentration of CecropinXJ was up to 0.4%,its inhibitory capacity could be comparable to commercial hand sanitizer(P>0.05).Further study elucidated that swabbed liquor included most of gramnegative bacteria through 16 SrDNA sequence analysis.These findings suggested that CecropinXJ could be used as a novel disinfector or cleanser for application potentially.

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