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当前位置: 首页 > 文章 > 新疆家蚕抗菌肽(Cecropin-XJ)特性的研究 新疆农业科学 2004,41 (1) 26-31
Position: Home > Articles > Studies on the Properties of Cecropin-XJ from Xinjiang Silkworm Xinjiang Agricultural Sciences 2004,41 (1) 26-31

新疆家蚕抗菌肽(Cecropin-XJ)特性的研究

作  者：
刘忠渊;张富春;蔡伦;赵干;单文娟;钟哲;王宾
单  位：
新疆大学生命科学与技术学院分子生物学重点实验室;新疆生物资源基因工程重点实验室
关键词：
新疆家蚕抗菌肽;抗菌活性;热稳定性;抑菌效价;人造胃液
摘  要：
探讨了新疆家蚕抗菌肽 (Cecropin -XJ)的抗菌特性。根据琼脂糖孔穴扩散法 ,检测抗菌肽的热稳定性、抑菌效价、对氨苄青霉素抗性菌的抑菌作用 ,检测抗菌肽对酸碱盐、人造胃液的耐受性及其抗菌谱。结果表明新疆家蚕抗菌肽 (Cecropin -XJ)具有很强的热稳定性、能够杀灭氨苄青霉素抗性菌、对酸碱盐、人造胃酸有一定的耐受性 ,其杀菌活力为 :1μg/ μl抗菌肽与 2 4 0 0 0单位的氨苄青霉素杀菌活力相当。新疆家蚕抗菌肽能够杀灭革兰氏阳性菌和革兰氏阴性菌 ,而对革兰氏阳性菌的抗菌活力高于革兰氏阴性菌 ,这一发现将为抗菌肽在农业、医疗卫生、畜牧业等方面的应用奠定基础 ,同时为深入研究抗菌肽作用机制提供了依据
译  名：
Studies on the Properties of Cecropin-XJ from Xinjiang Silkworm
作  者：
LIU ZHong-yuan~(1,2), ZHANG FU-chun~(1,2), CAI Lun~2, ZHAO Gan~2, SHAN Wen-juan~2, ZHONG ZHe~2, WANG Bin~2(1.Key Laboratory of Molecule Biology ,College of Life Science and Technology, Xinjiang,Urumqi 830000, China;2.Key Laboratory of Biological Resources and Genetic Engineering, Xinjiang University,Urumqi 830046,China)
关键词：
Cecropin-XJ Xinjiang silkworm;anti-bacterial activity;heat-stable;drug-resistant bacteria artificial gastric juice
摘  要：
The purpose of this study is to investigate the properties of recombinant Cecropin-XJ isolated from Xinjing silkworm and expressed in pichia yeast. By Agar Hole Pervasion tests, this recombinant Cecropin-XJ has exhibited an extreme heat-stable characteristic and the ability to kill ampicillin-resistant S. aureus. Moreover, we have observed that the Cecropin-XJ well tolerant to extreme acidic, basic, and high salt environments as well as resistant to 24 hours digestion by artificial gastric juice. The inhibition capability to S. aureus with 1μg/μl Cecropin-XJ is equal to 24 000U ampicillin. With a broad spectrum of antibacterial activities, the Cecropin-XJ is able to inhibit the Gram-positive bacteria and Gram-negative bacteria. However, it has exhibited a better inhibition to Gram-positive bacteria than that of Gram-negative bacteria. These findings could lead it to a broad applications for agriculture, medical, domestic animal and food industry. The further investigation of the antibacterial mechanism of Cecropin-XJ is needed.

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