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Position: Home > Articles > Study on the inhibition of Trichoderma sp.against Fusarium oxysporum f.sp.cubense in banana Journal of Fruit Science 2009,26 (2) 186-189+263

木霉菌对香蕉枯萎病菌的抑制作用

作  者:
钟小燕;梁妙芬;甄锡壮;赖进为;赖锡隆
单  位:
开平柏丽宝生物化学有限公司;三惠生物科技有限公司
关键词:
香蕉;木霉菌;香蕉枯萎病菌;抑制作用
摘  要:
实验室分离到1株对香蕉枯萎病菌(Fusarium oxysporum f.sp.cubens)有明显抑制作用的菌种,经18SrDNA鉴定为木霉属,命名为Trichoderma sp.G2。采用固体和液体培养法对菌株G2抑制香蕉枯萎病菌的生长进行了初步研究。结果表明,在对峙试验的平板上,7d香蕉枯萎病菌完全被G2的短绒状菌丝及分生孢子梗丛覆盖;在拮抗平板上,G2发酵液的平均抑制率约为90.4%,挥发性物质平均抑制率达68.3%,可见挥发性物质对抑制病原菌的生长起了重要的作用。在浓度为1.0×107cfu·mL-1香蕉枯萎病菌液体培养中,按体积比为20%接种G2,10d后镜检发现病原菌的菌丝及孢子大部分出现融解,表明菌株G2对香蕉枯萎病菌有明显的拮抗作用。
译  名:
Study on the inhibition of Trichoderma sp.against Fusarium oxysporum f.sp.cubense in banana
作  者:
ZHONG Xiao-yan1,LIANG Miao-fen1,ZHEN Xi-zhuang1,LAI Jin-wei1,2,LAI Xi-long1,2(1Kaiping PICO Bio-Technology CO.Ltd,Kaiping,Guangdong 529300 China;2Trinity Bio-Technology CO.Ltd,Kowloon,Hong Kong 999077 China)
关键词:
Banana;Trichoderma sp.;Fusarium oxysporum f.sp.cubens;Antagonism
摘  要:
A fungal strain,designated as G2,with remarkable ability of effective antagonist against Fusarium oxysporum f.sp.cubens,was isolated and identified as Trichoderma sp.based upon 18S rDNA gene analysis.With solid and liquid cul-tures,the inhibitive efficacy to the growth of Fusarium oxysporum f.sp.cubens was primarily studied.The experimental re-sults showed that the cells of Fusarium oxysporum f.sp.cubens were completely covered by short fiber-like hyphace and spore stem of G2 within 7 days in the dual culture plate,and in the antagonist plate,the average rate of inhibitory by the cul-ture solution of G2 was about 90.4%,the average rate of the inhibitory by volatile substance reached 68.3%.After 10 days' incubation with 20%(v/v) fungal strain G2,the melt of the pathogenic mycel and spore were observed in the liquid culture containing 1.0×107 cfu·L-1 Fusarium oxysporum f.sp.cubens,suggesting that the strain G2 can strongly inhibit the growth of Fusarium oxysporum f.sp.cubens.

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