单 位：
福建农林大学动物科学学院;福建省宁德市农业科学研究所
关键词：
中药饲料添加剂;断奶仔猪;一氧化氮;一氧化氮合酶;诱导型一氧化氮合酶
摘 要：
为研究中药精制的两种饲料添加剂Ⅰ(鱼腥草10%、黄芪20%、茯苓15%、刺五加10%、山楂15%、当归10%、厚朴10%和瞿麦10%)和添加剂Ⅱ(金银花10%、黄芪20%、茯苓15%、刺五加10%、山楂15%、当归10%、厚朴10%和瞿麦10%)对断奶仔猪血清一氧化氮(NO)含量及一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)活性影响,选择16窝胎次相近、日龄相同的杜长大三元仔猪,每窝9头左右,随机分成4组,每组4窝,分别为中药Ⅰ组、中药Ⅱ组、抗生素组和对照组。在相同的基础日粮情况下,前3个处理组分别添加中药饲料添加剂Ⅰ(4kg·t-1)、中药饲料添加剂Ⅱ(4kg·t-1)、金霉素(70g·t-1)和杆菌肽锌(30g·t-1)。在仔猪断奶后1、4、7、14、28d采血,分析断奶仔猪血清中NO含量、NOS和iNOS活性的变化。结果表明,断奶14d时,中药组供试猪血清NO含量分别比对照组提高了65.75%(P<0.05)和56.05%(P<0.05)。断奶28d时,中药Ⅰ组和中药Ⅱ组的NOS活性分别比对照组提高17%(P<0.05)和16.89%(P<0.05)。断奶1d时,中药Ⅰ组的iNOS活性较抗生素组降低6.4%(P>0.05),较对照组降低10%(P>0.05);中药Ⅱ组较对照组降低6.5%(P>0.05),但差异不显著。结果表明中药饲料添加剂能提高断奶仔猪血清NO含量和NOS活性,降低iNOS活性,对仔猪的胃肠道黏膜产生保护作用,缓解断奶应激,具有替代抗生素的潜能。
译 名：
Effects of Traditional Chinese Medicines on Serum NO and NOS/iNOS Activities in Weaned Piglets
作 者：
ZHANG Zhong-qiao;YE Yao-hui;ZHONG Lu-long;LEI Song-bo;College of Animal Science,Fujian Agriculture and Forestry University;Ningde Institute of Agricultural Sciences;
单 位：
ZHANG Zhong-qiao%YE Yao-hui%ZHONG Lu-long%LEI Song-bo%College of Animal Science,Fujian Agriculture and Forestry University%Ningde Institute of Agricultural Sciences
关键词：
traditional chinese medicine;;weaned piglets;;NO;;NOS;;iNOS
摘 要：
The effects of addition of two formulated herbal traditional Chinese medicines(TCM)in feed on serum NO and activities of NOS and iNOS of weaned piglets were investigated.TCM I was formulated to contain 10%Houttuynia cordata Thunb.,20% Astragalus membranaceus,15% Poria cocos,10% Acanthopanax senticosus,15% Crataegus pinnatifida Bunge,10% Angelica sinensis,10% Magnolia officinalis and 10% Dianthus superbus,whereas TCM II,10%Lonicera japonica Thunb.,20% Astragalus membranaceus,15% P.cocos,10%A.senticosus,15% C.pinnatifida Bunge,10% A.sinensis,10% M.officinalis and 10% D.superbus.From16litters(about 9heads per litter)of similar parity and age,the breastfeeding piglets were randomly divided into four groups,namely the TCM Group I,TCM Group II,Antibiotics Group and Control.TCM I at 4kg·t-1,TCM II at 4kg·t-1,chlortetracycline at 70kg·t-1 or bacitracin zinc at 30kg·t-1 was added to a same basal diet for the treatment groups.Blood samples of the piglets were taken at 1,4,7,14 and 28days after weaning to determine the serum NO and activities of NOS and iNOS.The contents of NO in TCM Group I and II piglets increased by65.75% and 56.05%,respectively,as compared to Control(P<0.05).After weaning for 28 days,the activity of NOS of piglets in TCM Group I was 17% and that of TCM Group II,16.89%,higher than Control(P<0.05).One day after weaning,the activity of iNOS in piglets in TCM Group I decreased 6.4%in comparison to Antibiotics Group(P>0.05)and declined 10% over Control(P>0.05),while that of TCM Group II decreased 6.5% over Control(P>0.05).The results indicated that the TCMs could increase serum NO and NOS activity and reduce iNOS activity protecting the gastrointestinal mucosa in weaned piglets.The addition effectively alleviated weaning stress on the piglets suggesting apotential for the TCMs to replace antibiotics administration for the purpose.
