Position: Home > Articles > Determination of Trichlorphon Residues in Aquatic Products Using UPLC-MS/MS
FOOD SCIENCE
2011,32
(8)
278-280
超高效液相色谱-质谱法测定水产品中敌百虫的含量
作 者：
查玉兵;杨春亮;林玲;李涛;程盛华
单 位：
中国热带农业科学院农产品加工研究所
关键词：
液相色谱-质谱/质谱联用;敌百虫;水产品;残留
摘 要：
建立敌百虫在水产品中残留量的超高效液相色谱-电喷雾质谱(UPLC-MS/MS)测定方法。用乙酸乙酯提取样品中的敌百虫,提取液经酸性氧化铝柱净化,经超高效液相色谱分离,用串联质谱测定,正离子多反应监测(MRM)模式检测,外标法定量。在0.02～0.8ng/mL范围内,敌百虫标准工作溶液的色谱峰面积与质量浓度成良好的线性关系,相关系数r=0.9992;水产品中敌百虫的添加回收率在89.0%～93.8%,相对标准偏差为2.1%～5.4%,检出限为1.0μg/kg。方法简便、快速、准确,保留时间为1.5min。
译 名：
Determination of Trichlorphon Residues in Aquatic Products Using UPLC-MS/MS
作 者：
ZHA Yu-bing,YANG Chun-liang,LIN Ling,LI Tao,CHENG Sheng-hua(1.Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001,China)
关键词：
UPLC-MS/MS;trichlorfon;aquatic products;residues
摘 要：
A UPLC-MS/MS method was developed to determine trichlorphon residues in aquatic products.Trichlorphon residues in samples were extracted into ethyl acetate,defatted on an acidic alumina solid phase extraction column,separated by UPLC,measured by MS/MS in positive ion multiple reaction monitoring mode,and quantified by external standard method. Over the range of 0.02 to 0.8 ng/mL,an excellent linear relationship(r=0.9992)between trichlorphon concentration and peak area was observed for the standard working solution of trichlorphon.The average spike recoveries for trichlorphon in blank samples ranged from 89.0%to 93.8%,with a RSD between 2.1%and 5.4%.The detection limit for trichlorphon was 1μg/kg. This method proved simple,rapid and accurate.
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