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月季花的快速繁育研究

作  者:
陈建雄;陈艳瑞;张艳敏
单  位:
新疆乌鲁木齐林业局林业处;中国科学院新疆理化技术研究所;乌鲁木齐市种苗场
关键词:
月季花;组织培养;快速繁育
摘  要:
[目的]为了快速繁育月季花的优良品种、保持品系的优良性状,最终实现月季花工厂化育苗。[方法]通过离体培养的方法对月季花进行组织培养基配方筛选试验。[结果]在月季组织培养快速繁育过程中,以腋芽为外殖体,在附加6-BA 2.0mg/L+NAA0.5~1.0mg/L+GA 1.0 mg/L的MS培养基上,分化率为95%,并生长成为丛生苗,分化的幼芽转移到1/2MS+NAA 0.5~1.0 mg/L或MS+6-BA 2.0 mg/L+IAA 1.0~1.5 mg/L的培养基上,诱导生根,形成了具有根、茎、叶的完整小植株,成活率达95%以上,极少数的试管苗还形成了花蕾。[结论]建立了迅速规模化生产月季花的组培方法。
译  名:
Study on Rapid Breeding of Chinese Rose
作  者:
CHEN Jian-xiong;Seedlings Field in Urumqi;
关键词:
Rosa chinansis;;Tissue culture;;Rapid propagation
摘  要:
[Objective]The aim was to fast breeding the fine varieties of Chinese rose and keep the fine character of its strain, finally realize the factory nursery of Chinese rose. [Method]In this paper, the tissue culture medium formula screening tests of Chinese rose have been carried out by using the method of in vitro culture. [Result]As a result, during the tissue culture and fast breeding process of Chinese rose, with axillary bud as outside body, the additional 6-BA 2.0 mg/L +NAA 0.5 1.0 mg/L +GA l. 0 mg/L on the MS culture medium, the bud differentiation rate of Chinese rose was 95%, and then it grew into clumps seedlings. Next,the germ of differentiation was transferred to the medium of 1/2 MS + NAA(0.5 1.0 mg/L) or MS + 6-BA 2.0mg/L + IAA l. 0-1.5 mg/L in order to be induced to grow root and form a complete small plant with roots,stems and leaves, the survival rate of them was more than 95%, and a handful of tube seedlings was also formed buds. [Conclusion]The rapidly transformation method to a large-scale production of Chinese rose was established.

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