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当前位置: 首页 > 文章 > 快速测定婴幼儿乳粉中的氯霉素残留 乳业科学与技术 2012,35 (6) 23-25
Position: Home > Articles > Rapid Determination of Residual Chloramphenicol in Infant Milk Powder by LC-MS-MS Journal of Dairy Science and Technology 2012,35 (6) 23-25

快速测定婴幼儿乳粉中的氯霉素残留

作  者：
王多娇;颜春荣;曲斌;卢剑;徐春祥
单  位：
江苏省产品质量监督检验研究院食品检测中心;江苏省畜产品质量检验测试中心
关键词：
婴幼儿乳粉;氯霉素;残留;LC-MS-MS
摘  要：
建立一种高效液相色谱-串联质谱法快速测定婴幼儿乳粉中氯霉素残留的方法。样品以D5-氯霉素为内标,碱化后,经乙酸乙酯提取、低温离心、氮吹浓缩,流动相复溶,采用高效液相色谱-串联质谱法测定。结果表明:在0.1～5μg/L范围内氯霉素呈良好的线性关系,高、中、低浓度回收率为80%～110%,精密度小于15%,定量限为0.1μg/kg。本方法快速、简便,适于乳粉中氯霉素残留的快速测定。
译  名：
Rapid Determination of Residual Chloramphenicol in Infant Milk Powder by LC-MS-MS
作  者：
WANG Duo-jiao1,YAN Chun-rong1,*,QU Bin2,LU Jian1,XU Chun-xiang1(1.Jiangsu Province Product Quality Supervision and Inspection Institute,Nanjing 210029,China;2.Jiangsu Province Livestock Products Quality Inspection and Test Center,Nanjing 210036,China)
关键词：
infant milk powder;chloramphenicol;residue;LC-MS-MS
摘  要：
A high performance liquid chromatography tandem mass spectrometric(HPLC-MS-MS) method was developed for rapid determination of chloramphenicol residue in infant milk powder.D5-Chloramphenicol was used as internal standard.Samples were alkalified,extracted with ethyl acetate and centrifuged at a low temperature.Afterwards,the supernatant was concentrated by nitrogen gas blowing and dissolved again in HPLC mobile phase before HPLC-MSMS analysis.An excellent linear relationship between peak area and chloramphenicol concentration was obtained over the concentration range of 0.1 to 5 μg/L.Average recoveries of chloramphenicol in a blank matrix across three spike levels ranged from 80% to 110%,and inter-batch and intra-batch precision(RSD) was less than 15%.The limit of quantification of this method was 0.1 μg/kg.This method was rapid,simple and suitable for rapid determination of chloramphenicol residue in infant milk powder.

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