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当前位置: 首页 > 文章 > 旋转中心设计优化土党参蛋白质提取工艺 上海农业学报 2017 (5) 93-98
Position: Home > Articles > Optimizing the protein-extracting process of lance asiabell roots by Box-Behnken design experiment Acta Agriculturae Shanghai 2017 (5) 93-98

旋转中心设计优化土党参蛋白质提取工艺

作  者：
陈致印;罗莹;刘广军;李魏巍;谭显胜
关键词：
土党参;蛋白质提取;Box-Behnken设计;响应面法
摘  要：
在单因素试验的基础上,选取pH、时间、液料比作为考察因素,利用Design-Expert 8.05b软件进行Box-Behnken旋转中心组合优化设计,响应面分析,确定微波提取土党参中蛋白质的最佳工艺参数为:提取时间6.41 min,pH 7.77,液料比26.1:1(mL/g),预测土党参中蛋白质提取率达到10.62%。试验证实该方法具有提取率高、耗能少的特点。
译  名：
Optimizing the protein-extracting process of lance asiabell roots by Box-Behnken design experiment
作  者：
CHEN Zhi-yin;LUO Ying;LIU Guang-jun;LI Wei-wei;TAN Xian-sheng;Hunan College of Humanities,Science and Technology;Hunan Provincial Collaborative Innovation Center for Farmland Weeds Control/Hunan Higher Education Key Laboratory of Harmless Application of Pesticides;
单  位：
Hunan College of Humanities,Science and Technology%Hunan Provincial Collaborative Innovation Center for Farmland Weeds Control/Hunan Higher Education Key Laboratory of Harmless Application of Pesticides
关键词：
Lance asiabell;;Protein extraction;;Box-Behnken design;;Response surface method
摘  要：
According to the single-factor experimental results of microwave assisted extraction of lance asiabell root proteins, the extracting time, liquid-material ratio and pH factors were selected, and their optimal technological parameters were determined through Box-Behnken design experiment and response surface analysis. The gained optimal technological parameters were 6. 41 min in extracting time, 26. 1:1 in liquidmaterial ratio( mL/g) and pH 7. 77, and the predicted extraction rate of lance asiabell root proteins could reach10. 62%. The experimental results showed that this optimizing method has such advantages as high extraction rate and low energy consumption.

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