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当前位置: 首页 > 文章 > 土党参多糖不同提取方法的比较研究 食品科学 2012,33 (18) 45-48
Position: Home > Articles > A Comparative Study of Different Extraction Methods for Polysaccharides from Campanumoea javanica Roots FOOD SCIENCE 2012,33 (18) 45-48

土党参多糖不同提取方法的比较研究

作  者：
吴兰芳;景永帅;张振东;杨娟
单  位：
贵州省中国科学院天然产物化学重点实验室
关键词：
土党参;多糖;热水浸提;超声波;微波;均匀设计
摘  要：
目的:比较3种提取方法对土党参原料多糖提取率的影响。方法:以土党参多糖提取率为考察指标,对热水浸提、超声波以及微波辅助3种提取方法进行均匀优化试验设计,对多糖提取率进行二次多项式逐步回归分析。结果:热水浸提法的优化条件为液料比25:1(mL/g)、温度70℃、提取时间60min,提取率28.05%;超声波提取法的优化条件为液料比50:1、功率210W、提取时间40min,提取率28.12%;微波法提取的优化条件为液料比15:1、功率900W、提取时间5min,提取率28.99%。结论:3种提取方法对多糖提取率影响差异不明显,但超声波和微波辅助提取较热水浸提具有省时的优点。
译  名：
A Comparative Study of Different Extraction Methods for Polysaccharides from Campanumoea javanica Roots
作  者：
WU Lan-fang,JING Yong-shuai,ZHANG Zhen-dong,YANG Juan(The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550002,China)
关键词：
Campanumoea javanica;polysaccharide;hot water extraction;ultrasound-assisted extraction;micro-wave-assisted extraction;uniform design
摘  要：
Objective:To compare the extraction efficiencies of polysaccharides from Campanumoea javanica roots by different extraction methods.Methods:Three different extraction methods were optimized by uniform design combined with second-order polynomial regression analysis for enhanced extraction efficiency.Results:The optimal hot water extraction conditions were material-to-liquid ratio of 1:25(g/mL),extraction temperature of 70 ℃ and extraction time of 60 min.Under these conditions,the maximum yield of polysaccharides was 28.05%.The optimal ultrasonic-assisted extraction conditions were material-to-liquid ratio of 1:50(g/mL),ultrasonic power of 210 W and extraction time of 40 min.Under these conditions,the maximum yield of polysaccharides was 28.12%.The optimal microwave-assisted extraction conditions were material-to-liquid ratio of 1:15(g/mL),microwave power of 900 W and extraction time of 5 min.Under these conditions,the maximum yield of polysaccharides was 28.99%.Conclusion:No significant difference was observed among three extraction methods.However,ultrasonic-assisted extraction and microwave-assisted extraction have a time-saving advantage over hot water extraction.

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