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当前位置: 首页 > 文章 > 高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究 食品科学 2013,34 (5) 202-206
Position: Home > Articles > Screening and Breeding of a Crypthecodinium cohnii Strain with High Productivity of Docosahexaenoic Acid and Optimization of Its Fermentation Conditions FOOD SCIENCE 2013,34 (5) 202-206

高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究

作  者：
孙中贯;刘咏;姚建铭;李俊;朱素梅
单  位：
中国科学院等离子体物理研究所;安徽科聚环保新能源有限公司;合肥工业大学生物与食品工程学院
关键词：
二十二碳六烯酸;亚硝基胍;隐甲藻;诱变育种;发酵条件
摘  要：
利用亚硝基胍对隐甲藻ATCC30556进行诱变处理,通过30℃高温培养初筛及摇瓶发酵复筛,得到二十二碳六烯酸(DHA)产量稳定提高的突变株LS1057,其DHA占总脂肪酸的含量从出发株的25.78%提高到32.02%。研究摇瓶发酵条件对突变株发酵的影响,结果表明:在初始pH6.5、温度26℃、转速150r/min的发酵条件下培养216h后,其生物量、总油脂产量及DHA产量分别达到14.06、3.14g/L和1.057g/L,分别比出发藻株提高了21.31%、69.73%和121.59%。
译  名：
Screening and Breeding of a Crypthecodinium cohnii Strain with High Productivity of Docosahexaenoic Acid and Optimization of Its Fermentation Conditions
作  者：
SUN Zhong-guan1,2,LIU Yong1,YAO Jian-ming3,LI Jun4,ZHU Su-mei4(1.School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China;2.College of Life Science,Zaozhuang University of Technology,Zaozhuang 277160,China;3.Institute of Plasma Physics,Chinese Academy of Sciences,Hefei 230031,China;4.Anhui Keju Green Energy Co.Ltd.,Hefei 230031,China)
关键词：
docosahexaenoic acid(DHA);nitrosoguanidine;Crypthecodinium cohnii;mutagenesis;fermentation conditions
摘  要：
In order to improve the productivity of docosahexaenoic acid,the mutagenesis was carried out through nitrosoguanidine(NTG) induction by using Crypthecodinium cohnii ATCC30556 as the original strain.The mutant LS1057 was selected through high-temperature(30 ℃) cultivation using docosahexaenoic acid content in total fatty acids as the evaluation index.The content of docosahexaenoic acid was increased from 25.78% to 32.02%.The fermentation conditions for LS1057 were optimized by single-factor tests to be pH 6.5,fermentation temperature of 26 ℃,shaking speed of 150 r/min and cultivation time of 216 h.Under the optimal conditions,the biomass of cultivated LS1057 could reach up to 14.06 g/L with total lipid of 3.14 g/L and docosahexaenoic acid of 1.057 g/L,which exhibited the enhancement by 21.31%,69.73% and 121.59% when its parent strain.

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