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当前位置: 首页 > 文章 > 二十二碳六烯酸对老年大鼠脑组织抗氧化和脂肪酸的影响 食品科学 2011,32 (13) 284-288
Position: Home > Articles > Effect of DHA on Antioxidation and Fatty Acids in Aged Rat Brain FOOD SCIENCE 2011,32 (13) 284-288

二十二碳六烯酸对老年大鼠脑组织抗氧化和脂肪酸的影响

作  者：
蒋利和;马博;谢正轶;杨志荣
单  位：
广西大学科技处;广西医科大学人体解剖学教研室;百色学院化学与生命科学系;四川大学生命科学学院
关键词：
二十二碳六烯酸;抗氧化;脂肪酸
摘  要：
目的:研究二十二碳六烯酸(DHA)改善老年大鼠抗氧化的可能机制。方法:动物分为青年组(7月龄青年雄性大鼠)、老年组(20月龄雄性大鼠)、DHA低剂量组(灌喂20月龄雄性大鼠DHA 180mg/(kg.d)42d)、DHA高剂量组(灌喂20月龄雄性大鼠360mg/(kg.d)42d),处死大鼠后测定脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活力、丙二醛(MDA)含量,然后用气相色谱-质谱联用分析法分析脑脂肪酸组成及含量,最后用薄层层析和磷钼酸法分析磷脂构成。结果:与老年大鼠相比,灌喂DHA的各组大鼠的脑中SOD、GSH-Px、CAT活性显著提高,同时灌喂DHA减少MDA的含量;对脑脂肪酸和磷脂的分析,表明灌喂DHA显著提高脑DHA含量,增加总n-3脂肪酸/总n-6脂肪酸和DHA/AA(二十碳四烯酸)的比率,显著降低AA含量,还能显著提高磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇、磷脂酰丝氨酸及神经鞘磷脂的含量。结论:DHA显著提高自然衰老大鼠的脑组织抗氧化能力,其机制可能与改变脑脂肪酸组成有关。
译  名：
Effect of DHA on Antioxidation and Fatty Acids in Aged Rat Brain
作  者：
JIANG Li-he1,2,MA Bo2,3,Xie Zheng-yi4,YANG Zhi-rong2,(1.The Science & Technology Division,Guangxi University,Nanning 530004,China;2.School of Life Science,Sichuan University,Chengdu 610064,China;3.Department of Chemistry and Life Science,Baise College,Baise 533000,China;4.Department of Human Anatomy,Guangxi Medical University,Nanning 530021,China)
关键词：
docosahexaenoic acid(DHA);antioxidation;fatty acids
摘  要：
Objective:To explore the antioxidant mechanisms of DHA in the brain of aged rats.Methods:Wistar male rats were randomly divided into four groups including 7-month-old adult group,20-month-old aged group,and low-dose and high-dose DHA-treated 20-month-old aged groups at the respective doses of 180 mg/(kg·d) and 360 mg/(kg·d)(administered by gavage for 42 consecutive days).At the end of the administration period,all rats were sacrificed and the activities of superoxide dismutase(SOD),gtutathione peroxidase(GSH-Px) and catalase(CAT) and the level of malondialdehyde(MDA) were assayed in brain.In addition,the fatty acid profile in brain was analyzed by gas chromatography-mass spectrometry,and the phospholipid composition was also analyzed by thin layer chromatography and phosphomolybdate methods.Results:Compared with the aged group,the activities of SOD,GSH-Px and CAT in the brain of the DHA-treated groups were significantly increased and MDA content was significantly decreased.Fatty acid analysis showed that DHA-treated rats exhibited an obvious increase in the ratio of total n-3 fatty acids to total n-6 fatty acids and the ratio of DHA to arachidonic acid(AA) and an obvious reduction in AA level.It was also revealed that DHA could improve the levels of phosphatidylcholine,phosphatidylethanolamine,phosphatidylinositol,phosphatidylserine and sphingomgelins in the brain of rats.Conclusion:DHA can obviously improve antioxidant capacity in aged rats and its antioxidant mechanism may be due to DHA-induced change of fatty acid profile in the brain.

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