单 位：
陕西师范大学运动生物学研究所;商洛学院生物医药工程系
关键词：
杜仲提取物;肝组织;标志酶活性;运动训练;自由基代谢;运动能力
摘 要：
【目的】探讨杜仲提取物(Eucommia ulmoides Oliver extract,EUOE)对运动训练大鼠肝组织的保护作用以及肝脏标志酶、运动能力的影响。【方法】将24只SD雄性健康大鼠随机均分为安静对照组、运动对照组和运动加药组,运动对照组及运动加药组大鼠进行6周的大强度耐力跑台训练,期间运动加药组大鼠每天灌胃EUOE2.06g/kg,其他2组灌服相同体积的生理盐水,第6周最后1天在运动对照组和运动加药组大鼠力竭运动后,取血清及肝组织样品,测试大鼠肝组织总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、胞浆SOD(Cu,Zn-SOD)、线粒体SOD(Mn-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,丙二醛(MDA)、还原性谷胱甘肽(GSH)含量以及血清谷丙转氨酶(GPT)活性,并记录力竭时间。【结果】服用杜仲提取物组大鼠力竭运动后肝组织T-AOC、T-SOD及Cu,Zn-SOD、Mn-SOD、GSH-Px、CAT活性均显著(P<0.05)或极显著(P<0.01)高于运动对照组,均显著(P<0.05)或极显著(P<0.01)低于安静对照组;MDA含量显著(P<0.05)低于运动对照组,极显著(P<0.01)高于安静对照组;肝组织GSH含量显著(P<0.05)高于运动对照组;血清GPT活性显著低于运动对照组(P<0.05)。与运动对照组比较,运动加药组大鼠运动至力竭的时间延长23.09%。【结论】杜仲提取物可以改善长时间大强度耐力运动大鼠肝组织的氧化应激水平,保护肝组织抗氧酶活性,维持运动训练大鼠肝组织结构和功能,提高大鼠的整体机能,延缓运动疲劳。
译 名：
Protection of Eucommia ulmoides Oliver extract on the oxidative damage of rat's liver tissue for taking exercise training
作 者：
WANG Xin-jun1,WANG Yi-min2,XIONG Zheng-ying2(1 Department of Biological Medicine Engineering,Shangluo University,Shangluo,Shaanxi 726000,China; 2 Institute of Sports Biology,Shaanxi Normal University,Xi'an,Shaanxi 710062,China)
关键词：
Eucommia ulmoides extract;liver;activity of marker enzyme;sports training;free radicals metabolism;athletic ability
摘 要：
【Objective】 This study aimed to investigate the influence of eucommia Eucommia ulmoides on liver tissues,liver marker enzymes and exercise capacity of rats taking exercise training.【Method】 24 healthy male SD rats were randomly divided into 3 groups,sedentary control group,exercise control group and exercise with medicine control group.Rats of exercise group and exercise with medicine control group attended a 6-week exhaustive endurance treadmill training.A model of exercise training,test total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD),cytoplasmic(Cu,Zn-SOD),mitochondrial(Mn-SOD),catalase(CAT),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),reduced glutathione(GSH) content and serum alanine aminotransferase(GPT) activity of liver tissues was established.【Result】 T-AOC,T-SOD,Cu,Zn-SOD,Mn-SOD,GSH-Px and CAT activities in liver tissues of rats taking extract of E.ulmoides and attending exhaustive exercise were significantly(P<0.05) or very significantly(P<0.01) higher than that of the exercise control group and significantly(P<0.05) or very significantly(P<0.01) lower than that of the sedentary control group.Contents of MDA and GPT were notably(P<0.05) lower than that the exercise control group and notably(P<0.05) or very notably(P<0.01) higher than that the sedentary control group.Hepatic GSH content was significantly higher than that of the exercise control group(P<0.05),and serum GPT activity was significantly lower than that of the exercise control group(P<0.05) and very significantly lower than that of the sedentary control group(P<0.05).Exercise with medicine control group had 23.09% prolonged exhaustive exercise time compared with exercise control group.【Conclusion】 E.ulmoides extract can improve the level of oxidative stress in liver tissue during long high-intensity endurance exercise,protect antioxidant activity of liver tissue,and maintain the liver tissue structure and function of rats taking exercise training,so as to improve the overall function of rats and slow motion fatigue.
