中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 培养方法对灵芝菌丝体胞内三萜产量和抗肿瘤活性的影响 上海农业学报 2014,30 (2) 33-37
Position: Home > Articles > Influences of culture methods on the yield and antineoplastic activity of intracellular triterpene of Ganoderma lucidum mycelia Acta Agriculturae Shanghai 2014,30 (2) 33-37

培养方法对灵芝菌丝体胞内三萜产量和抗肿瘤活性的影响

作  者：
骆军鑫;张劲松;贾薇;冯娜;杨炎;唐庆九;刘艳芳;章慧
单  位：
农业部南方食用菌资源利用重点实验室
关键词：
灵芝;菌丝体;三萜产量;培养法;抗肿瘤活性
摘  要：
采用液体震荡-静置两阶段培养法进行灵芝液体深层发酵培养,与液体震荡培养法相比,灵芝菌丝体胞内总三萜含量提高了1.7倍左右,总三萜产量提高了2.5倍左右。研究表明:在静置过程中,上层为白色菌丝体,下层为黄色菌球;上层菌丝体总三萜含量是下层菌丝体的3倍,总三萜产量是下层菌丝体的4倍。另外在静置阶段采用培养瓶不会影响灵芝菌丝体总三萜含量,且上层菌丝体的生物量提高了50%,从而大大提高了上层菌丝体总三萜产量。高效液相色谱(HPLC)分析表明:上层菌丝体中含有的三萜组分种类比下层菌丝体多;细胞试验表明:上层菌丝体的醇提取物在作用体积浓度为50μg/mL时对肝癌细胞HepG2的抑制率达到62.91%。
译  名：
Influences of culture methods on the yield and antineoplastic activity of intracellular triterpene of Ganoderma lucidum mycelia
作  者：
LUO Jun-xin;ZHANG Jin-song;JIA Wei;FENG Na;YANG Yan;TANG Qing-jiu;LIU Yan-fang;ZHANG Hui;National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;Shanghai Ocean University;
关键词：
Ganoderma lucidum;;Mycelium;;Triterpene yield;;Culture method;;Antineoplastic activity
摘  要：
Compared with the liquid shaking flask culture in deep fermentation of Ganoderma lucidum,the two-stage method of shaking culture and static culture could not only make the mycelium biomass not decrease but also increase the total triterpene content and the total triterpene yield by about 1.7 times and about 2.5 times respectively.It was found that during the static culture the upper stratum was white mycelia,the substratum was yellow pellets,the total triterpene content of the upper mycelia was 2 times higher than that of the lower mycelia,and the total triterpene yield of the upper mycelia was 3 times higher than that of the lower mycelia.Besides,at the static culture stage the used flask had no effect on the total triterpene content of the mycelia but the upper mycelium biomass increased by 50%,thus greatly improving the total triterpene yield of the upper mycelia.The analysis by HPLC showed that the kinds of intracellular triterpene components of the upper mycelia were more than those of the lower mycelia,and the cell test indicated that when the upper mycelia's ethanol extraction was at 50 μg/mL,the rate of inhibiting HepG2 cells reached 62.91%.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46