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当前位置: 首页 > 文章 > 拟南芥RING E3连接酶AtASSR1在盐胁迫响应中的功能初探 四川农业大学学报 2017 (3) 381-388
Position: Home > Articles > Preliminary Study on Function of Arabidopsis RING E3 Ligase AtASSR1 in Salt Stress Response Journal of Sichuan Agricultural University 2017 (3) 381-388

拟南芥RING E3连接酶AtASSR1在盐胁迫响应中的功能初探

作  者：
杨亮;常伟;李志;苗明军;李跃建;刘志斌;李旭锋;杨毅
单  位：
四川省农业科学院园艺研究所蔬菜种质与品种创新四川省重点实验室;四川大学生命科学学院生物资源与生态环境教育部重点实验室
关键词：
拟南芥;AtASSR1;E3连接酶;盐胁迫
摘  要：
【目的】构建拟南芥AtASSR1转基因株系,研究并初步揭示RING E3连接酶AtASSR1在盐胁迫应答中的作用。【方法】利用农杆菌介导的花序浸染法,成功获得AtASSR1基因插入的纯合转基因株系,并通过检测盐胁迫处理前后,转基因株系及野生型、atassr1突变体植株中生理生化指标来初步揭示AtASSR1的功能。【结果】通过检测盐胁迫处理前后,不同基因型株系的H_2O_2、过氧化氢酶活性、丙二醛的含量,发现转基因株系中的过氧化氢酶活性最低,而积累的H_2O_2与丙二醛含量最多;分析脯氨酸含量发现,盐胁迫处理后突变体株系的脯氨酸含量积累最多。【结论】E3连接酶AtASSR1可能在介导植物响应盐胁迫等非生物胁迫的应答中发挥重要生理功能。
译  名：
Preliminary Study on Function of Arabidopsis RING E3 Ligase AtASSR1 in Salt Stress Response
作  者：
YANG Liang;CHANG Wei;LI Zhi;MIAO Ming-jun;LI Yue-jian;LIU Zhi-bin;LI Xu-feng;YANG Yi;Horticulture Research Institute,Sichuan Academy of Agricultural Sciences,Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province;Key Laboratory of Bio-resources and Eco-environment,Ministry of Education,College of Life Science,Sichuan University;
关键词：
Arabidopsis thaliana;;AtASSR1;;E3 ligase;;salt stress
摘  要：
【Objective】The AtASSR1 transgenic plants were constructed and studied to investigate the function of RING E3 ligase AtASSR1 plays an important effect on the responses of Arabidopsis to salt stress.【Method】AtASSR1 homozygous transgenic lines were successfully constructed through the Agrobacterium tumefaciens-mediated transformation. This studies was performed to preliminarily reveal the function of AtASSR1 by analyzing the physiological and biochemical indexes in wild-type,atassr1 mutant,and transgenic lines treated with or without Na Cl.【Results】The results show that the transgenic lines have the lowest activity of CAT and the highest content of H_2O_2 and MDA by analyzing the content of H_2O_2,catalase(CAT),and Malondialdehyde(MDA)in different genotypes of lines treated with or without Na Cl. The atassr1 mutant accumulates the highest content of proline after salt stress by analyzing the content of proline in different genotype lines.【Conclusion】All of these results suggested that AtASSR1 may play an important physiological function in plant abiotic stress responses.

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