中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 同时检测动物性食品中6种β-受体激动剂的化学发光免疫分析方法的建立 肉类研究 2017 (10) 36-40
Position: Home > Articles > Simultaneous Determination of 6 β-agonists in Animal-Derived Food by Chemiluminescence Immunoassay MEAT RESEARCH 2017 (10) 36-40

同时检测动物性食品中6种β-受体激动剂的化学发光免疫分析方法的建立

作  者：
王建军;熊雅婷;秦誉;杨恒峰;张鹏坤
单  位：
贺州市水产畜牧兽医局;北京维德维康生物技术有限公司;保定市动物产品质量检验监测中心;抚顺市动物疫病预防控制中心
关键词：
化学发光免疫分析;动物性食品;β-受体激动剂
摘  要：
建立同时测定动物性食品中6种β-受体激动剂(克伦特罗、西马特罗、溴布特罗、班布特罗、马布特罗和西布特罗)残留的化学发光免疫分析方法。结果表明:6种β-受体激动剂在猪肉样品中的检测限(limit of detection,LOD)分别为0.48、1.96、0.41、0.29、0.42、0.87μg/kg,在羊肉样品中的LOD分别为0.37、1.78、0.43、0.38、0.31、0.96μg/kg;样本的平均添加回收率为66.7%~106.4%,且批内、批间相对标准偏差(relative standard deviation,RSD)均小于15%。在最优条件下,西马特罗的50%抑制质量浓度(half maximal inhibitory concentration,IC_(50))为0.254μg/L,克伦特罗、溴布特罗、班布特罗、马布特罗和西布特罗与西马特罗的交叉反应率分别为438.0%、470.0%、434.0%、561.0%和198.0%。同时,比较化学发光免疫分析方法和液相色谱-串联质谱(liquid chromatographic-tandem mass spectrometric,LC-MS/MS)法的检测结果,表明化学发光免疫分析方法具有较高的准确度和可靠性。因此,化学发光免疫分析方法能够实现动物性食品中多种β-受体激动剂药物残留的快速检测,且灵敏度较高。
译  名：
Simultaneous Determination of 6 β-agonists in Animal-Derived Food by Chemiluminescence Immunoassay
作  者：
WANG Jianjun;XIONG Yating;QIN Yu;YANG Hengfeng;ZHANG Pengkun;Baoding Animal Products Quality Inspection and Monitoring Center;Beijing WDWK Biotechnology Co.Ltd.;Hezhou Municipal Bureau of Fishery and Veterinary Medicine;Fushun Animal Disease Prevention and Control Center;
单  位：
Baoding Animal Products Quality Inspection and Monitoring Center%Beijing WDWK Biotechnology Co.Ltd.%Hezhou Municipal Bureau of Fishery and Veterinary Medicine%Fushun Animal Disease Prevention and Control Center
关键词：
chemiluminescence immunoassay;;animal-derived food;;β-agonist
摘  要：
In this paper,a chemiluminescence immunoassay(CLIA)for simultaneously detecting 6 β-agonist drugs residues in animal-derived food was established.The limits of detection(LODs)for clenbuterol,cimaterol,bromobuterol,bambuterol,mabuterol and sibuterol in pork were 0.48,1.96,0.41,0.29,0.42 and 0.87 μg/kg,respectively,and in mutton were 0.37,1.78,0.43,0.38,0.31 and 0.96 μg/kg,respectively.Mean recoveries from spiked samples ranged from 66.7% to 106.4%,and the intra-assay and inter-assay relative standard deviation(RSD)values were less than 15%.The half maximal inhibitory concentration(IC_(50))of cimaterol was 0.254 μg/L under optimum conditions,and its cross-reaction rates with clenbuterol,bromobuterol,bambuterol,mabuterol and sibuterol were 438.0%,470.0%,434.0%,561.0% and 198.0%,respectively.The developed immunoassay was more accurate and reliable than liquid chromatographic-tandem mass spectrometric(LC-MS/MS).Therefore,chemiluminescence immunoassay proved to be an effective analytical technique for monitoring β-agonist drug residues in animal-derived food owing to its high sensitivity.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46