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Position: Home > Articles > Determination of Ractopamine Residues in Swine Urine by Direct Competitive Chemiluminescence Immunoassay China Animal Husbandry & Veterinary Medicine 2009,36 (12) 200-203

化学发光直接竞争免疫分析法检测猪尿中莱克多巴胺残留

作  者:
高彬文;张素霞;梁永红;刑保松
单  位:
中国农业大学动物医学院;河南农业科学院畜牧兽医研究所
关键词:
化学发光免疫分析;莱克多巴胺;猪尿
摘  要:
本试验研究建立了猪尿中莱克多巴胺(Ractopamine,RAC)残留的直接竞争化学发光免疫检测方法。合成了莱克多巴胺和辣根过氧化物酶的偶联物(RAC-HRP),方法的检测限为0.09 ng/mL,空白猪尿中添加浓度为0.5、10和100 ng/mLRAC时,检测范围为0.3~157.0 ng/mL;回收率为76.3%~87.6%,批内变异系数为10.0%~12.2%,批间变异系数为14.2%~15.2%。
译  名:
Determination of Ractopamine Residues in Swine Urine by Direct Competitive Chemiluminescence Immunoassay
作  者:
GAO Bin-wen1,2,ZHANG Su-xia2,LIANG Yong-hong1,XING Bao-song1(1.Institute of Animal Husbandry and Veterinary Research Science,Henan Agriculture Science,Zhengzhou 450002,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)
关键词:
chemiluminescence immunoassay;Ractopamine;swine urine
摘  要:
A method was developed for the determination of RAC residues in swine urine by direct competitive chemiluminescence immunoassay(CLIA).The limit of detection was 0.09 ng/mL and the detection ranged from 0.3 ng/mL to 157.0 ng/mL.At the fortified levels of 0.5 ng/mL,10 ng/mL and 100 ng/mL,mean recoveries ranged from 76.3% to 87.6%.The intra-assay variations were 10.0% to 12.2% and the inter-assay variations were 14.2% to 15.2%.

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