中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 香蕉两种主要病毒多重PCR检测方法的建立 园艺学报 2006,33 (4) 845-848
Position: Home > Articles > Development of a Multiplex PCR Protocol for the Detection of Two Viruses of Banana Acta Horticulturae Sinica 2006,33 (4) 845-848

香蕉两种主要病毒多重PCR检测方法的建立

作  者：
彭军;王国芬;黄俊生;代鹏;谢玉萍;李伟东
单  位：
中国热带农业科学院环境与植物保护研究所;中华人民共和国海南出入境检验检疫局
关键词：
香蕉;香蕉束顶病毒;黄瓜花叶病毒;病毒检测;RT-PCR
摘  要：
根据香蕉束顶病(Bananabunchytopvirus,BBTV)和香蕉花叶心腐病(Cucumbermosaicvirus,CMV)的复制酶基因、外壳蛋白基因序列分别设计特异性引物对,在BBTV单一PCR和CMVRT-PCR优化体系的基础上,建立了可同时检测BBTV、CMV的多重PCR检测方法。此方法可以特异地从感染BBTV和CMV的样品中扩增出2条带,BBTV(748bp)和CMV(557bp)。扩增产物序列测定结果表明,BBTV扩增产物与GenBank中其他分离物的核苷酸序列同源性为91%~99%,CMV扩增产物与GenBank中其他分离物的核苷酸序列同源性为93%~98%。
译  名：
Development of a Multiplex PCR Protocol for the Detection of Two Viruses of Banana
作  者：
Peng Jun~1,Wang Guofen~1,Huang Junsheng~ 1,2* ,Dai Peng~1,Xie Yuping~1,and Li Weidong~3 ( ~1Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China ; ~2State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101,China; ~3Hainan Entry-exit Inspection and Quarantine Bureau of Peoples Republic of China, Haikou, Hainan 570311, China )
关键词：
Banana; Banana bunchy top virus(BBTV);Cucumber mosaic virus(CMV) ;Virus detection;RT-PCR
摘  要：
A multiplex RT-PCR was developed for the simultaneous detection of two viruses of banana and plantain (Musa spp.). Two sets of specific primer pairs were designed according to the gene sequence of Banana bunchy top virus (BBTV) replicase region and Cucumber mosaic virus (CMV) coat protein region, respectively. Based upon the establishment of the optimized PCR and RT-PCR detection of BBTV and CMV, the multiplex RT-PCR which can simultaneously detect the two banana viruses was developed. It was shown that all samples infected with BBT and CMV could be amplified simultaneously by multiplex RT-PCR, and yield two specific bands of BBTV (748 bp) and CMV (557 bp) visualized by agarose gel electrophoresis. Sequence analysis of the amplified products showed high homology of BBTV (91%-99%) and CMV (93%-98%) with the sequences of BBTV and CMV in GenBank.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46