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Position: Home > Articles > Polymorphism Analysis on Partial Sequence of Pig Obese Gene of Different Breeds by PCR-SSCP Journal of Northeast Agricultural University(English Edition) 2006,13 (2)

Polymorphism Analysis on Partial Sequence of Pig Obese Gene of Different Breeds by PCR-SSCP

作者：

SONG Yuefen;YU Hao;YANG Xiuqin;LIU Di

单位：

;College of Animal Science and Technology, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC

关键词：

breed;ob gene;pcr-sscp;polymorphisms

摘要：

Polymorphisms of porcine ob exon 1 and exon 2 among different breeds including Landrace, Duroc, Min pig, Yorkshire pig, double-muscled Yorkshire, Sanjiang pig, wild boar and cross bred pig were analyzed by PCR-SSCP in the current study. Three pairs ofprimers according to the ob cDNA sequence obtained from GenBank database were designed to amplify the first two exons, which were then genotyped by SSCP. The T to C transversion was found in exon 2, which resulted in 3 genotypes named AA, AB and BB, respectively in these different porcine breeds. There was only genotype of BB in the Min pig, while no allele B was detected in double-muscled Yorkshire, and the 3 genotypes all existed in other breeds. There was significant difference on the genotype frequencies in various breeds. There was a trend that the frequency of allele A was positively associated with muscle ratio distribution on the one hand, and on the other hand, it was linked to the selected direction. So the allele A could be used as a selective marker of high muscle ratio in pig breeding.

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Polymorphism Analysis on Partial Sequence of Pig Obese Gene of Different Breeds by PCR-SSCP

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东北农业大学学报(英文版)

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