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当前位置: 首页 > 文章 > 正交试验优化大孔吸附树脂分离纯化杜仲绿原酸 食品科学 2013,34 (16) 23-26
Position: Home > Articles > Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin FOOD SCIENCE 2013,34 (16) 23-26

正交试验优化大孔吸附树脂分离纯化杜仲绿原酸

作  者：
熊硕;崔丽;刘仲华;张盛;肖文军
单  位：
湖南农业大学园艺园林学院;湖南农业大学园艺园林学院,国家植物功能成分工程技术研究中心
关键词：
杜仲叶;绿原酸;大孔吸附树脂;分离纯化
摘  要：
以杜仲叶60%乙醇提取液为原料,研究NKA-2、NKA-9、LS-600、HZ806、HZ906、HPD100、HPD500、S-8八种大孔吸附树脂对绿原酸的静态吸附与解吸效果,优化最优树脂分离纯化杜仲叶绿原酸的工艺技术参数。结果表明:NKA-2树脂对绿原酸具有较好的吸附与解吸性能,在pH3的条件下静态吸附量为86mg/g,解吸率达93.23%;当主要考虑绿原酸得率时,最优工艺参数为上样质量浓度4mg/mL、上样流速4BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速为3BV/h、洗脱体积为2BV,在该工艺参数条件下,绿原酸得率为83.14%,纯度为28.91%;当主要考虑绿原酸纯度时,最优工艺参数为上样质量浓度4mg/mL、上样流速4BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速为3BV/h、洗脱体积为1.5BV,在该工艺参数条件下,绿原酸得率为79.62%,纯度为39.38%。
译  名：
Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin
作  者：
XIONG Shuo,CUI Li,LIU Zhong-hua,ZHANG Sheng,XIAO Wen-jun(National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients,College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China)
关键词：
Eucommia ulmoidues leaves;chlorogenic acid;macroporous resin;separation and purification
摘  要：
Different types of macroporous adsorption resin,including NKA-2,NKA-9,LS-600,HZ806,HZ906,HPD100,HPD500 and S-8 were compared for their static adsorption and desorption capacities for chlorogenic acid in 60% ethanol extract of Eucommiaulmoidues leaves.NKA-2 was found to have a stronger ability to adsorb and desorb chlorogenic acid and the optimal operating parameters for purifying chlorogenic acid from the ethanol extract using NKA-2 were determined.The static adsorption quantity of chlorogenic acid on NKA-2 and desorption percentage were 86 mg/g and 93.23%,respectively.The optimal process conditions that provided maximum chlorogenic acid recovery(83.14%;purity 28.91%) were determined as 4 mg/mL,4 BV/h,5 BV,80%,3 BV/h and 2 BV for sample concentration,loading flow rate,loading volume,desorbent concentration,desorption flow rate,and desorbent volume,respectively,and whereas those for maximum chlorogenic acid purity(39.38%;recovery 79.62%) were 4 mg/mL,4 BV/h,5 BV,80%,3 BV/h and 1.5 BV,respectively.

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