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当前位置: 首页 > 文章 > 杜仲叶树脂分离纯化产物体外抗氧化活性研究 食品科学 2011,32 (1) 27-30
Position: Home > Articles > in vitro Antioxidant Activity Evaluation of Elution Fractions of Ethanol Extract from Eucommia ulmoides Leaves on XDA-8 Type Macroporous Resin FOOD SCIENCE 2011,32 (1) 27-30

杜仲叶树脂分离纯化产物体外抗氧化活性研究

作  者：
董文宾;刘迪;杨津;许先猛
单  位：
陕西科技大学生命科学与工程学院;陕西科技大学化学与化工学院
关键词：
杜仲叶;树脂;分离纯化;抗氧化活性;自由基
摘  要：
为了研究杜仲叶的体外抗氧化活性,杜仲叶粗提物被XDA-8型大孔吸附树脂吸附后,依次用不同体积分数的乙醇溶液进行梯度洗脱,得到4种分离纯化产物,测定了4种产物对.OH、O2.及DPPH自由基清除能力,同时测定了各产物中多酚和总黄酮的质量分数。结果表明:4种产物均具有良好的清除.OH、O2.及DPPH自由基能力,且对各自由基的清除能力呈量效关系;产物抗氧化活性与总黄酮和多酚成分有关,且多酚类物质起主导作用;收集不同乙醇体积分数的洗脱液可以得到不同成分含量的抗氧化剂。
译  名：
in vitro Antioxidant Activity Evaluation of Elution Fractions of Ethanol Extract from Eucommia ulmoides Leaves on XDA-8 Type Macroporous Resin
作  者：
DONG Wen-bin1,2,LIU Di2,YANG Jin1,XU Xian-meng1(1.College of Life Science and Engineering,Shaanxi University of Science and Technology,Xi'an 710021,China; 2.College of Chemistry and Chemical Industry,Shaanxi University of Science and Technology,Xi'an 710021,China)
关键词：
Eucommia ulmoides leaves;resin;separation and purification;antioxidant activity;free radical
摘  要：
The crude ethanol extract from the leaves of Eucommia ulmoides was applied to XDA-8 type macroporous resin,followed by gradient elution with different concentrations(20%,40%,60% and 80%) of ethanol,and four fractions were obtained.The abilities of the four fractions to scavenge hydroxyl,superoxide anion and DPPH free radicals were measured.Meanwhile,the contents of polyphenols and total flavonoids in them were determined.It was found that all the four fractions had excellent scavenging effect on hydroxyl,superoxide anion and DPPH free radicals in a dose-dependent way.This biological activity might be related to total flavonoids and polyphenols,and polyphenols play a leading role.After eluting XDA-8 type macroporous resin with different concentrations of ethanol,eluates were obtained as antioxidants with different chemical composition.

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