中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 雏番鸭胰腺型鸭1型甲肝病毒分离鉴定及VP1基因分析 福建农业学报 2012,27 (9) 37-42
Position: Home > Articles > Identification and Sequence Analysis of Duck Hepatitis A Virus Type 1 Isolated from Muscovy Duckling with Pancreatitis Fujian Journal of Agricultural Sciences 2012,27 (9) 37-42

雏番鸭胰腺型鸭1型甲肝病毒分离鉴定及VP1基因分析

作  者：
傅光华;陈红梅;黄瑜;施少华;彭春香;江斌;程龙飞;万春和;傅秋玲;林建生;林芳
单  位：
福建省农业科学院畜牧兽医研究所
关键词：
鸭甲肝病毒;胰腺型;雏番鸭;VP1衣壳蛋白
摘  要：
采集自胰腺发黄或出血的雏番鸭病料经RT-PCR检测为鸭1型甲肝病毒阳性后,接种11日龄非免疫番鸭胚获得一株病毒(命名为MPZJ1206)。该株病毒在不同温度下均不凝集鸡、鸭和绵羊红细胞;鸭胚中和试验表明蚀斑纯化毒可被经典的鸭1型甲肝病毒高免血清特异性所中和。该株病毒对7日龄雏番鸭的致死率为38.5%,病死鸭剖检病变与临床发病鸭相同,且从病死鸭脏器中回收分离到的病毒经鉴定仍为鸭1型甲肝病毒。应用鸭1型甲肝病毒VP1基因特异性引物从该株病毒克隆获得的714bp的基因片段,与经典鸭1型甲肝病毒分离株Du/CH/LGD/111239VP1基因的同源性最高,为99.1%,经遗传进化分析表明该株病毒属鸭1型甲肝病毒谱系。以上结果表明,雏番鸭胰腺炎是由鸭1型甲肝病毒感染所致,这一新病型明显不同于经典的鸭1型甲肝病毒感染引起的肝脏出血,鉴于此,建议将本试验分离株命名为胰腺型鸭1型甲肝病毒。
译  名：
Identification and Sequence Analysis of Duck Hepatitis A Virus Type 1 Isolated from Muscovy Duckling with Pancreatitis
作  者：
FU Guang-hua,CHEN Hong-mei,HUANG Yu,SHI Shao-hua,PENG Chun-xiang,JIANG Bin, CHENG Long-fei,WAN Chun-he,FU Qiu-ling,LIN Jian-sheng,LIN Fang(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences, Fuzhou,Fujian 350013,China)
关键词：
duck hepatitis A virus;pancreatitis;muscovy duckling;VP1 protein
摘  要：
Samples came from Muscovy ducklings with yellowed or hemorrhagic pancreatitis were positive for duck hepatitis A virus-1(DHAV-1) by RT-PCR.A virus strain(named as MPZJ1206) was abtained from Virus-containing allantoic fluid after inoculation of embryonated Muscovy duck eggs with DHAV-1-positive samples.The isolate shows no hemagglutinating activity with chickens,ducks or sheep red blood cells at different temperature.Plaque purified virus was specifically neutralized by anti-DHAV-1 hyperimmune serum.Seven-day-old Muscovy ducklings inoculated with the virus showed a mortality rate of 38.5%,and yielded the same pathological characteristics as those of clinical onset duck had.The virus strain also recovered from the pancreas of Muscovy duckling died in the trials.A 714-bp fregment gene was abtained by using a set of primers aimed at VP1 ORF gene,and shared 99.1% nucleotide sequence identity with that of classical DHAV-1 strain Du/CH/LGD/111239.Phylogenetic analysis showed that MPZJ1206 strain lay in the viral lineage of DHAV-1.The above results indicate that pancreatitis in Muscovy ducklings was caused due to the infection of duck hepatitis A virus type 1,and this pathotype is distincted from hemorrhagic hepatitis caused by classical duck hepatitis A virus,so it advised that the virus isolated in this study could be named as pancreatitis-type DHAV-1.

相似文章

导出引用
计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46