当前位置: 首页 > 文章 > 鸭甲肝病毒与新型呼肠孤病毒复合RT-PCR检测方法的建立 中国动物传染病学报 2015,23 (3) 50-54
Position: Home > Articles > MULTIPLEX RT-PCR FOR DETECTION OF DUCK HEPATITIS A VIRUS AND NOVEL DUCK REOVIRUS Chinese Journal of Animal Infectious Diseases 2015,23 (3) 50-54

鸭甲肝病毒与新型呼肠孤病毒复合RT-PCR检测方法的建立

作  者:
孙晓军;王晓艺;韩宏宇;迟海华;杨宏禹;于可响
单  位:
山东农业大学动物科技学院;山东省蓬莱市大柳行畜牧兽医工作站;山东省农业科学院家禽研究所山东省家禽疫病诊断与免疫重点实验室;山东省烟台市姜格庄兽医站
关键词:
鸭甲肝病毒;新型鸭呼肠孤病毒;复合RT-PCR;鉴别诊断
摘  要:
根据鸭甲肝病毒(Duck hepatitis A virus,DHAV)的3D基因序列和新型鸭呼肠孤病毒(Novel Duck reovirus,NDRV)的S3基因序列,分别设计了1对特异性引物,通过条件优化建立了鉴别DHAV和NDRV的复合RT-PCR方法。该方法对DHAV和NDRV的最低检出量均为100 copies,而对番鸭呼肠孤病毒(Muscovy duck reovirus,MDRV)、鸭瘟病毒(Duck plague virus,DPV)、鸭坦布苏病毒(Duck Tembusu virus,DTMUV)和鸭副粘病毒(Duck paramyxo virus,NDV)的检测结果均为阴性。临床样品的检测结果显示,该方法是一种快速鉴别诊断DHAV和NDRV感染的有效手段。
译  名:
MULTIPLEX RT-PCR FOR DETECTION OF DUCK HEPATITIS A VIRUS AND NOVEL DUCK REOVIRUS
作  者:
SUN Xiao-jun;WANG Xiao-yi;HAN Hong-yu;CHI Hai-hua;YANG Hong-yu;YU Ke-xiang;Key Laboratory of Poultry Disease Diagnosis and Immunology of Shandong Province, Institute of Poultry, Shandong Academy of Agricultural Sciences;Jianggezhuang Veterinary Station of Yantai City,Shandong Province;College of Animal Science Veterinary Medicine, Shandong Agricultural University;Daliuhang Veterinary Station of Penglai City, Shandong Province;
关键词:
Duck hepatitis A virus;;Novel duck reovirus;;multiplex reverse transcription polymerase chain reaction;;differential diagnosis
摘  要:
A multiplex reverse transcription polymerase chain reaction(RT-PCR) assay for detecting Duck hepatitis A virus(DHAV) and Novel duck reovirus(NDRV) was developed using two primers based on 3D gene of DHAV and S3 gene of NDRV. The detection limit of the RT-PCR assay was 100 copies for both viruses. Mean while, the method had no reaction with Muscovy duck reovirus(MDRV), Duck plague virus(DPV), Duck Tembusu virus(DTMUV) and Duck paramyxo virus(NDV). The results of testing clinical samples showed the assay was a useful technique for differential detection of DHAV and NDRV.

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