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当前位置: 首页 > 文章 > 海狸鼠卵巢卵母细胞体外成熟培养前后的超微结构研究 黑龙江动物繁殖 1997 (2) 3-6
Position: Home > Articles > Study on Ultrastructure of Nutria Ooctes Before and After Maturation in Vitro Heilongjiang Journal of Animal Reproduction 1997 (2) 3-6

海狸鼠卵巢卵母细胞体外成熟培养前后的超微结构研究

作  者：
孟庆刚;孙凤俊;邴印忠;王继先;谭景和
单  位：
中国科学院黑龙江农业现代化研究所;东北农业大学动物科学系;黑龙江省巴彦县畜牧局
关键词：
海狸鼠;卵母细胞;体外成熟;超微结构
摘  要：
实验用光学显微镜和透射电镜观察了海狸鼠卵巢卵母细胞在体外成熟培养前后的形态及超微结构。结果表明.在成熟培养前,海狸鼠卵母细胞的微绒毛多插入透明带,有的退化;皮层颗粒多数散在分布于皮质区;线粒体多数成行排列分布于皮质区或细胞核周围,线粒体多为椭圆形,多嵴;细胞核位于皮质区,核膜有时打褶。成熟培养后,微绒毛从透明带中撤出,倒伏在质膜上;皮层颗粒多成行排列于质膜下,线粒体开始散在分布于胞质中;高尔基体和粗面内质网消失;成熟的卵母细胞生发泡破裂.有第一极体排出。这些结果说明,海狸鼠成熟卵母细胞的超微形态学特征与其它哺乳动物基本一致。
译  名：
Study on Ultrastructure of Nutria Ooctes Before and After Maturation in Vitro
作  者：
Meng Qinggang et al.(Nortbeast Agricultural University 150030)
关键词：
nutria;oocyte;in vitro maturation;ultrastructure
摘  要：
The ultrastructural feature and morphology of the nutria (Myocaster coypus) oocytes before and after in vitro maturation were studied with optical miccoscope and electron microscope. Before culture, most of the microvilli on oocyte surface penetrated into the zona pellucida, some of them degenerated. Most of the cortical granules distributed in the cortical region. Most of mitochondria distributed in line in the cortical region or around the nucleus, and most of them were oval. The nucleus was located in the cortical region. The membrane of nucleus which was folded sometimes. After culture, microvilli of the oocyte withdrew from the zona pellucida, and lay on the membrane of oocyte. Most of the cortical granules distributed in line under the membrane of the oocyte. Mitochndria distributed in the ooplasm. Golgi's complexes (Go.) and rough endoplasmic reticulum (RER) disapeared. The germ viscles of the matured oocytes broke down, and first polar body discharged in the perivitelline space. These results indicated that the ultrastructural features of nutria oocytes were basically similar to the oocytes of other mannal animals.

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