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当前位置: 首页 > 文章 > 牡丹愈伤组织增殖及褐化研究 广东农业科学 2019 (6) 30-36
Position: Home > Articles > 牡丹愈伤组织增殖及褐化研究 Guangdong Agricultural Sciences 2019 (6) 30-36

牡丹愈伤组织增殖及褐化研究

作  者：
程雨飞;朱向涛;季雯;洪尔蔓;林芯;范贞;张俊丽
单  位：
潍坊职业学院农林科技学院;浙江农林大学暨阳学院;临沂市园林局
关键词：
牡丹;愈伤组织;继代增殖;预培养;褐化;赤霉素
摘  要：
[目的]提高牡丹愈伤组织增殖倍数和质量,减轻增殖过程中的褐化现象,为牡丹愈伤组织增殖提供途径.[方法]以牡丹胚轴、子叶愈伤组织为材料,对不同添加物组合及浓度、不同预培养时间等方法手段进行探究.[结果]牡丹胚轴愈伤组织继代增殖最佳培养基组合是PVP 1.0 g/L+IAA 0.2 mg/L+TDZ 1.0mg/L+GA3 1.0 mg/L,增殖倍数为5.75;子叶愈伤组织继代增殖的最佳培养基组合是PVP 2.0 g/L+IAA 0.5mg/L+TDZ 0.5 mg/L+GA3 1.0 mg/L,增殖倍数为5.50.采用预培养30 d的外植体,可有效提高增殖倍数并减轻褐化.1.0 mg/L GA3有利于抑制愈伤组织增殖过程中的褐化现象,并促进愈伤组织增殖.呈淡黄绿色表面附着絮状物且质地松软的愈伤组织在继代增殖过程中不易褐化,且中后期体积增殖变化明显.[结论]牡丹愈伤组织增殖中加入1.0 mg/L GA3和采用预培养30 d的愈伤组织,可以获得较高的增殖倍数并有效抑制褐化,呈淡黄绿色且质地松软的愈伤组织是良好的愈伤组织增殖状态.
作  者：
CHENG Yufei;ZHU Xiangtao;JI Wen;HONG Erman;LIN Xin;FAN Zhen;ZHANG Junli;Jiyang College, Zhejiang Agricultural and Forestry University;Linyi Bureau of Landscape;College of Agriculture and Forestry Science and Technology, Weifang Vocational College;
单  位：
CHENG Yufei%ZHU Xiangtao%JI Wen%HONG Erman%LIN Xin%FAN Zhen%ZHANG Junli%Jiyang College, Zhejiang Agricultural and Forestry University%Linyi Bureau of Landscape%College of Agriculture and Forestry Science and Technology, Weifang Vocational College
关键词：
Paeonia suffruticosa;;callus;;subculture multiplication;;pre-culture;;browning;;GA3
摘  要：
【Objective】 The study was to improve the multiplication times and quality of peony callus to reduce the browning in the process of proliferation and provide a way for the proliferation of peony callus. 【Methods】 Different additive combinations and concentrations, different pre-culture time and other methods were explored with peony cotyledon and hypocotyl callus as materials. 【Results】 The best medium combinations for the multiplication of peony hypocotyl callus and cotyledon callus are PVP 1.0 mg/L+IAA 0.2 mg/L+TDZ 1.0 mg/L+GA3 1.0 mg/L and PVP 2.0 mg/L+IAA 0.5 mg/L+TDZ 0.5 mg/L+GA3 1.0 mg/L, and the proliferation times are 5.75 and 5.50, respectively. The use of explants that have been pre-cultured for 30 d can effectively increase the proliferation times and reduce browning. GA3 1.0 mg/L is beneficial to inhibit browning during callus proliferation and promoted callus proliferation. The callus in the form of light yellowish green floccule on the surface with spongy texture is not prone to brown during subculture multiplication, with the volume proliferation changing significantly in the middle and late stages. 【Conclusion】 The addition of 1.0 mg/L of GA3 and the use of the callus that has been pre-cultured for 30 d in the process of peony callus proliferation can achieve higher proliferation times and effectively inhibit browning. Light yellowish green and spongy callus is a sound condition of callus proliferation.

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