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当前位置: 首页 > 文章 > 6种病毒抑制剂对烟草花叶病毒的抑制作用及其机制初探 河南农业科学 2011,40 (5) 110-113
Position: Home > Articles > Inhibition Activity of Six Virus Inhibitors against Tobacco Mosaic Virus and Their Primary Mechanism Journal of Henan Agricultural Sciences 2011,40 (5) 110-113

6种病毒抑制剂对烟草花叶病毒的抑制作用及其机制初探

作  者：
谢鑫;桑维钧;张新强;倪云跃;杨茂发;郦黔江
单  位：
贵州大学;贵州道元科技有限公司
关键词：
烟草花叶病毒;病毒抑制剂;病程相关蛋白基因;基因表达;抑制机制
摘  要：
采用半叶枯斑法测定6种病毒抑制剂对烟草花叶病毒(TMV)的活体治疗、保护和钝化作用,并用半定量RT-PCR检测药剂诱导烟叶中病程相关蛋白基因PR-5的表达情况。结果表明:有效成分为宁南霉素的金珠蔬美对TMV有较高的活体治疗和保护效果,在250 mg/L时枯斑抑制率分别达到了67.24%、54.55%;关于药剂对TMV的活体钝化作用,金毒冠氨基寡糖素的抑制率最高,5 mg/L时平均抑制率为60.31%。6种药剂处理烟草K326后,PR-5基因表达呈现不同程度地上调,表明它们均能诱导烟草产生对TMV的抗性。
译  名：
Inhibition Activity of Six Virus Inhibitors against Tobacco Mosaic Virus and Their Primary Mechanism
作  者：
XIE Xin1,SANG Wei-jun1,2,ZHANG Xin-qiang1,NI Yun-yue1,YANG Mao-fa1,LI Qian-jiang2 (1.Agricultural College of Guizhou University,Guiyang 550025,China;2.Guizhou Daoyuan Technology Limited Corporation,Guiyang 550014,China)
关键词：
Tobacco mosaic virus;Virus inhibitors;Pathogenesis related proteins gene;Gene expression;Inhibition mechanism
摘  要：
A half-leaf method was used to detest protective,curative and inactivation effects of six virus inhibitors against Tobacco mosaic virus(TMV),and the expression of pathogenesis-related protein gene PR-5 was also determined by semi-quantitative RT-RCR.The results showed that Jinzhushumei consisting of Ningnanmycin provided the best curative and protective effects,with relative inhibition rates of 67.24% and 54.55%,respectively.The inactivation effect of Jinduguan chito-amino-oligosaccharids was the best,with relative inhibition rate of 60.31%.PR-5 gene expression increased in different proportions in tabacco K326 leaves treated by six virus inhibitors,which indicated that these virus inhibitors induced the disease resistance of tobacco against TMV.

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