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当前位置: 首页 > 文章 > 同步检测为害非洲菊3种病毒的多重RT-PCR方法研究 上海农业学报 2009,25 (2) 26-30
Position: Home > Articles > Study on multiplex RT-PCR method to synchronously detect 3 kinds of viruses damaging Gerbera jamesonii Bolus Acta Agriculturae Shanghai 2009,25 (2) 26-30

同步检测为害非洲菊3种病毒的多重RT-PCR方法研究

作  者：
张丽莉;杨翠云;于翠;袁文辉;马青
单  位：
西北农林科技大学植保学院与陕西省农业分子生物学重点实验室;上海出人境检验检疫局;上海大地种苗有限公司
关键词：
番茄斑萎病毒;烟草脆裂病毒;烟草花叶病毒;多重RT-PCR;非洲菊
摘  要：
以接种番茄斑萎病毒(TSWV)的烟草和侵染烟草脆裂病毒(TRV)及烟草花叶病毒(TMV)的非洲菊为试验材料,建立了同步检测非洲菊上述3种病毒的多重RT-PCR方法。结果表明,建立的多重RT-PCR方法可一次性扩增出3种病毒的DNA条带,可以从RNA含量1 mg的带毒叶片中检测到混合病毒;同时对单一病毒RT-PCR的灵敏度检测表明:最低可以从RNA含量分别为1 mg、10μg和100ng的带毒材料中检测到TSWV、TRV和TMV。
译  名：
Study on multiplex RT-PCR method to synchronously detect 3 kinds of viruses damaging Gerbera jamesonii Bolus
作  者：
ZHANG Li-li1,YANG Cui-yun2,YU Cui2,YUAN Wen-hui3,Ma Qing1(1 College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agricultureand Northwest A & F University,Yangling 712100,China;2Shanghai Entry-Exit Inspection andQuarantine Bureau,Shanghai 200135,China;3Shanghai Dadi Seedling Co.Ltd.,Shanghai 201317,China)
关键词：
Tomato spotted wilt virus;Tobacco rattle virus;Tobacco mosaic virus;Multiplex RT-PCR;Gerbera jamesonii Bolus
摘  要：
Nicotiana tabacum inoculated with tomato spotted wilt virus(TSWV) and Gerbera jamesonii infected with tobacco rattle virus(TRV) and tobacco mosaic virus(TMV) were used as test materials,and a multiplex RT-PCR method was established to synchronously detect the above 3 kinds of viruses.The test results indicated that the DNA bands of all the viruses could be amplified just once by the established method,and the mixed viruses could be detected from 1 mg RNA-containing leaf with viruses.The sensitivity analysis of the RT-PCR method showed that the TSWV,TRV and TMV could be detected in a virus-infected material containing RNA of at least 1 mg,10 μg and 100 ng respectively.The established multiplex RT-PCR method had high specificity and sensitivity and could greatly reduce the detecting time.

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