当前位置: 首页 > 文章 > 香榧主要栽培品种的RAPD分析 园艺学报 2002,29 (1) 69-71
Position: Home > Articles > Molecular Identification of Main Cultivars of Torreya grandis by RAPD Markers Acta Horticulturae Sinica 2002,29 (1) 69-71

香榧主要栽培品种的RAPD分析

作  者:
谭晓风;胡芳名;张党权;周煦惠;杨伟
单  位:
中南林学院经济林育种与栽培国家林业局重点实验室
关键词:
香榧;品种;RAPD标记;分子鉴别;分子分类
摘  要:
以香榧 8个主要栽培品种无性系的种子胚乳DNA为研究材料 ,利用 2 3种多态型明显的引物进行了RAPD分析。结果表明 :8个品种之间在DNA水平上表现出较大的异质性 ;根据RPAD特异分离谱带确定了各品种的标记基因型 ,以此可实现对香榧无性系品种DNA水平上的鉴别 ;以标记基因型为依据对香榧 8个品种进行系统聚类分析 ,结果与传统形态学分类中的两大类基本吻合
译  名:
Molecular Identification of Main Cultivars of Torreya grandis by RAPD Markers
作  者:
Tan Xiaofeng,Hu Fangming,Zhang Dangquan,Zhou Xuhui,and Yang Wei (The Key laboratory of Non wood Forest Tree Breeding and Cultivation of Forestry Ministry,Zhuzhou 412006)
关键词:
Torreya grandis ;Cultivars;RAPD marker;Molecular identification;Molecular classification
摘  要:
The endosperm DNA samples of the seeds of a same individual plant of eight main cultivars (clones) of Torreya grandis are analyzed with RAPD technology by using 23 primers that have distinct polymorphism?The result indicated that there was a relatively large difference between the eight varieties at the level of DNA molecule; the marker genotype of varieties was established according to particularly isolated bands in the experiments; the clone varieties of Torreya grandis can be identified at the level of DNA molecule according to the marker genotype; the eight varieties of Torreya grandis were analyzed systematically with a assembling analysis technology, and the analytic result tallied with the two classes of traditional morphological classification on the whole.

相似文章

计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊