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当前位置: 首页 > 文章 > 邻二氮菲分光光度法测定稻米中的植酸含量 湖南农业大学学报(自然科学版) 2023,49 (3) 377-382
Position: Home > Articles > Determination of phytic acid content in rice by 1,10-phenanthroline spectrophotometry Journal of Hunan Agricultural University(Natural Sciences) 2023,49 (3) 377-382

邻二氮菲分光光度法测定稻米中的植酸含量

作  者：
贺为毅;王葵;彭晓赟;黄明春;萧浪涛;刘登友;王若仲
单  位：
湖南省衡阳县农业农村局;湖南农业大学化学与材料学院;湖南农业大学生物科学技术学院
关键词：
稻米;植酸;分光光度法;邻二氮菲
摘  要：
建立了一种邻二氮菲分光光度法测定稻米植酸含量的方法:加入过量Fe3+与植酸生成 4∶1PA(Fe)4沉淀,再利用盐酸羟胺将剩余Fe3+还原为Fe2+,以邻二氮菲为显色剂,采用分光光度法定量检测Fe2+,从而实现对稻米植酸含量的检测.对测定体系的pH和反应时间进行优化,分析无机磷酸对植酸含量测定的影响,验证方法的重复性和精确度.结果表明:在溶液pH为2.5、反应时间为30 min条件下,可准确测定的植酸浓度为6.7×10-7~2.5×10-5 mol/L,低浓度和高浓度植酸测定工作曲线的R2均大于 0.99;无机磷酸浓度低于 2.0×10-5 mol/L时,游离的无机磷酸对植酸检测无影响;对 6.7×10-7~2.5×10-5 mol/L的标准样品进行 5次平行试验,回收率为 93.30%~104.67%,RSD为 1.25%~7.34%.利用该方法测定的黄华占、金穗 408、Kitaake、日本晴等 4个品种的稻米植酸含量分别为5.42、4.72、6.40、5.68 mg/g,RSD为 1.23%~7.60%.
译  名：
Determination of phytic acid content in rice by 1,10-phenanthroline spectrophotometry
作  者：
HE Weiyi;WANG Kui;PENG Xiaoyun;HUANG Mingchun;XIAO Langtao;LIU Dengyou;WANG Ruozhong;College of Bioscience and Biotechnology, Hunan Agricultural University;Hunan Provincial Key Laboratory of Phytohormones and Growth Development;Agriculture and Rural Bureau of Hengyang County;School of Chemistry and Materials Science, Hunan Agricultural University;
关键词：
rice;;phytic acid;;spectrophotometry;;1,10-phenanthroline
摘  要：
In this study,a 1,10-phenanthroline spectrophotometry for the determination of phytic acid content in rice was established.The protocol included three steps,first add excess Fe~(3+)and phytic acid to form 4∶1 PA(Fe)_(4 )precipitation,then use hydroxylamine hydrochloride to reduce the remaining Fe~(3+)to Fe~(2+),after that use 1,10-phenanthroline as a color developer to quantitatively detect Fe~(2+)by spectrophotometry,and finish the detection of phytic acid content in rice.The pH and reaction time of the determination system was optimized,and analyze the effect of inorganic phosphoric acid on the determination of phytic acid content,the reproducibility and accuracy of the method were verified.The results showed that the phytic acid concentration could be accurately determined in the range of 6.7×10~(-7)-2.5×10~(-5 )mol/L under the pH 2.5 and reaction time was 30 min,and the R~(2 )of the working curves for the determination of phytic acid at low and high concentrations were greater than 0.99.The free inorganic phosphate did not affect the detection of phytic acid when the inorganic phosphate concentration was lower than 2.0×10~(-5 )mol/L.The recoveries were 93.30%-104.67%and the RSD of 1.25%-7.34%for five parallel tests on standard samples of 6.7×10~(-7)-2.5×10~(-5 )mol/L.The phytic acid contents of the rice of four varieties,including Huanghuazhan,Jinsuo408,Kitaake and Nipponbare,determined by this method were5.42,4.72,6.40,5.68 mg/g,the RSD were 1.23%to 7.60%.

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