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当前位置: 首页 > 文章 > 苹果渣果胶多糖的分离纯化与抗氧化活性研究 农业工程学报 2008 (1) 218-222
Position: Home > Articles > Purification and antioxidative activity of apple pectin from apple pomace Transactions of the Chinese Society of Agricultural Engineering 2008 (1) 218-222

苹果渣果胶多糖的分离纯化与抗氧化活性研究

作  者：
马惠玲;盛义保;张丽萍;于修烛
单  位：
西北农林科技大学食品与工程学院;西北农林科技大学生命科学学院;睢宁县林业局;合肥工业大学应用物理系
关键词：
苹果果胶;分离纯化;DPPH·自由基清除;抗氧化活性;红外光谱
摘  要：
为了选择合适的果胶提取工艺及探明产品组分与功效之间的关系,指导果胶的合理应用,该文将采用高温(100℃)酸提工艺、中温(87℃)酸提工艺从苹果渣中提取得到的3种果胶多糖半纯品即高温酸提果胶沉淀部分和溶解部分、中温酸提果胶经离子交换柱依次用水、0.1mol/L NaCl、0.3mol/L NaCl进行洗脱得到多糖纯品,比较了这些纯品与半纯品对二苯代苦基肼基自由基(DPPH·)的清除作用及半抑制浓度(IC50)。结果表明,各半纯品经离子交换柱均洗脱出3个纯品组分;半纯品对DPPH·自由基的清除作用均高于纯品;三种半纯品对DPPH·的半抑制浓度依次为0.6、1.8、4.2mg/mL;同一半纯品的水冼纯品组分的半抑制浓度均低于盐洗组分。高温酸提工艺提取的果胶多糖得率和活性均高于中温酸提工艺。前者提取的两个半纯品于菜油乳液中表现出浓度与抗氧化活性的量-效关系,其中高温酸提果胶沉淀部分的作用强度达到2,6-二叔丁基-对甲酚(BHT)的2倍,有效使用浓度可望低至0.1g/kg。中温酸提果胶的作用则不明显。红外光谱分析得出,抗氧化活性较高的苹果果胶组分可能为复合有酚类化合物的氨基多糖。
译  名：
Purification and antioxidative activity of apple pectin from apple pomace
作  者：
Ma Huiling 1 ,Sheng Yibao 2 ,Zhang Liping 3 ,Yu Xiuzhu 4 (1.College of life science,Northwestern Agriculture and forestry University,712100,China;2.Department of Applied physics,HeFei University of Industry,Anhui,230009,China;3.Forestry bureau of Xinin county,Xuzhou Jiangsu,221200;4.College of Food Science and Engineering Northwestern Agriculture and forestry University,Yangling,712100,China)
关键词：
apple pectin;Isolation;DPPH·scavenging;Antioxidative activity;Fourier transform infrared spectroscopy(FTIR)
摘  要：
In order to choose the proper technology for the extraction of pectin and elucidate the relationship between compositions and biological activity of pectin for the behalf of rational use of pectin,three half-pure polysaccharides,which were precipitate part of 100℃-extracting pectin,supernant part of 100℃-extracting pectin and 87℃-extracting pectin from apple pomace were isolated with ion exchange column.Purities were collected by eluting the column with water,0.1 mol/L NaCl and 0.3 mol/L NaCl,in turn.The scavenging activity to free radical diphenylpicrylhydrazyl(DPPH·)of these half-pure and pure products and their half inhibitory concentration(IC50)were measured.The results show that each of the half-pure polysaccharide is separated into three kinds of pure compositions through ion exchange chromatogram.The DPPH·scavenging activity of each half-pure polysaccharide is higher than that of its pure compositions.Corresponding IC50of the three half-pure polysaccharides is 0.6、1.8、4.2mg/mL,respectively.The IC50of water-eluting fraction was higher than that of salt-eluting fractions for the same half-purity.The yield and activity of 100℃-extracting is higher than that of 87℃-extracting pectin.The 100℃-extracting pectin in the rape oil presented dosage-effect relationship between concentration and antioxidation activity. The antioxidative activity of the precipitate part of 100℃-extracting pectin is two times that of 2,6-Di-tert-butyl-4-methylphenol(BHT)and its best use concentration is lower of 0.1g/kg.No obvious effect was observed in 87℃-extracting pectin.Fourier transform infrared spectroscopy(FTIR)of each purity suggests that there is amino polysaccharide combined with phenolic compounds in apple pectin with stronger antioxidative activity.

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