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当前位置: 首页 > 文章 > 克伦特罗荧光免疫层析试纸条的制备及特性研究 中国畜牧兽医 2015,42 (10) 2606-2611
Position: Home > Articles > Preparation and Properties Analysis of Clenbuterol Fluorescent Immunochromatographic Strip China Animal Husbandry & Veterinary Medicine 2015,42 (10) 2606-2611

克伦特罗荧光免疫层析试纸条的制备及特性研究

作  者：
李静宇;何小维;刘晓云;李文美;邓建超
单  位：
广州万孚生物技术股份有限公司;华南理工大学轻工与食品学院
关键词：
克伦特罗;荧光免疫层析法;试纸条;快速检测
摘  要：
本研究旨在制备性能优异的克伦特罗(clenbuterol,CLB)荧光免疫层析快速定量检测试纸条,为实现其产业化应用奠定基础。采用荧光胶乳微粒为标记物,以抗原包被浓度、膜干燥时间、混匀反应时间、反应温度及层析时间为单因素优化制备了CLB荧光免疫层析试纸条,并对该试纸条的检测限、特异性、临床准确度及添加回收率、精密度和稳定性进行了评价。最佳制备条件:抗原包被浓度2.0mg/mL,膜干燥时间5d,混匀反应时间1min,反应温度25℃,层析时间15min。评价结果显示,试纸条检测限可达0.35μg/L;CLB与其他8种同类药物交叉反应率均小于0.8%,特异性良好;各批添加回收率在80%~120%之间,准确度达到要求;不同CLB浓度下变异系数(CV)均小于5%,符合精密度标准;此外,稳定性试验验证了该试纸条稳定性良好。
译  名：
Preparation and Properties Analysis of Clenbuterol Fluorescent Immunochromatographic Strip
作  者：
LI Jing-yu;HE Xiao-wei;LIU Xiao-yun;LI Wen-mei;DENG Jian-chao;College of Light Industry and Food Sciences,South China University of Technology;Wondfo Biotech Co.,Ltd.;
关键词：
clenbuterol;;fluorescent immunochromatography;;strip;;rapid detection
摘  要：
This study was aimed to prepare excellent fluorescence immunochromatographic strip for clenbuterol(CLB)which could achieve the purpose of rapid and quantitative detection and laid a foundation for its industrialization.The strip was prepared through single factor experiments using latex particles as a fluorescent label.The preparation process was optimized with CLB concentration,drying time,mixing reaction time,reaction temperature and chromatography time.And the properties of this strip including detection limit,specificity,clinical accuracy and added recovery,degree of precision and stability were investigated.Optimum modification conditions were shown as following:CLB concentration was 2.0mg/mL,drying time was 5d,mixing reaction time was 1min,reaction temperature was 25 ℃,chromatography time was 15 min.The detection limit was up to 0.35μg/L and the cross rate between CLB and other eight similar drugs were less than0.8% which demonstrated the good specificity.The added recoveries of each batch were between80% and 120% which met the requirements.The coefficient of variation(CV)under different concentrations of CLB was less than 5% which met the precision standard.Besides,the stability of the experiment also showed that the test strip had good stability.

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