关键词：
罂粟壳;快速检测方法;食品安全;液相色谱—质谱法;竞争抑制免疫层析法
摘 要：
目的:建立食品中罂粟壳成分的快速检测方法,为罂粟壳成分的检测提供依据。方法:对监督执法中抽检的20份样品中的5种罂粟壳成分应用竞争抑制免疫层析快速检测法进行检测,然后利用液相色谱—质谱法对阳性样品进行检测验证。结果:经液相色谱—质谱法和竞争抑制免疫层析法检测,20份样品中有2份检测阳性、18份阴性,合格率90%,竞争抑制免疫层析法阳性检出率100%。结论:竞争抑制免疫层析法快速、便捷,干扰因素少,阳性检出率高,值得应用推广。
译 名：
Application of Fast Detection Metheds of Component of Poppy Shell in Food Supervision
作 者：
ZHU Zhen-hua;ZHU Shi-xin;CHEN Lu;ZHU Jia-xian;Health Supervision Institute of Kunshan City;
关键词：
poppy shell;;rapid detection method;;food safety;;Liquid Chromatography-Mass Spectrometry(LC-MS);;Competitive Inhibition Assay
摘 要：
【Objective】To establish a rapid method for compenents of poppy shell to provide reliable basis for the detection. 【Method】Totally 20 samples were detected by Competitive Inhibition Assay,and 2 positive samples were validated by Liquid Chromatography-Mass Spectrometry. 【Result】After Liquid Chromatography-Mass Spectrometry and Competitive Inhibition Assay,2 samples were in 20 samples,and the qualified rate was 90%,positive detection rate by the Competitive Inhibition Assay was 100%. 【Conclusion】The Competitive Inhibition Assay was rapid,convenient and less interference,positive detection rate was high,which was worth to be popularized in food supervision.
