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当前位置: 首页 > 文章 > 传染性法氏囊病病毒VP2蛋白B细胞抗原表位免疫小鼠试验 华北农学报 2010,25 (2) 233-235
Position: Home > Articles > Mice Immunization with the B-cell Epitopes on the VP2 Protein of Infectious Bursal Disease Virus Acta Agriculturae Boreali-Sinica 2010,25 (2) 233-235

传染性法氏囊病病毒VP2蛋白B细胞抗原表位免疫小鼠试验

作  者：
肖治军;张改平;杨汉春;杨艳艳;赵东;李学伍;邓瑞广;柴书军;邢广旭;杨继飞
单  位：
河南省农业科学院;中国农业大学
关键词：
IBDV;VP2蛋白;合成肽;抗原表位;免疫
摘  要：
为检测Ⅰ型IBDVVP2蛋白线性B细胞抗原表位肽PJ14和PJ5的免疫原性,将人工合成并纯化的PJ14和PJ5分别与牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联;将与BSA偶联的PJ14和PJ5分别免疫BALB/c小鼠,然后以与OVA偶联的PJ14和PJ5作为间接酶联免疫吸附试验抗原,分别测定免疫鼠血清中抗相应多肽的抗体。结果显示,免疫小鼠产生了抗PJ14和PJ5的抗体,抗体持续期达21周。表明PJ14和PJ5具有免疫原性。
译  名：
Mice Immunization with the B-cell Epitopes on the VP2 Protein of Infectious Bursal Disease Virus
作  者：
XIAO Zhi-jun1,ZHANG Gai-ping1,YANG Han-chun2,YANG Yan-yan1,ZHAO Dong1,LI Xue-wu1,DENG Rui-guang1,CHAI Shu-jun1,XING Guang-xu1,YANG Ji-fei1(1.Key Laboratory of Animal Immunology of the Ministry of Agricultural,Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China)
关键词：
IBDV;VP2 protein;B-cell epitope;Peptide synthesis;Immunization
摘  要：
The peptides of the linear B-cell epitopes PJ14 and PJ5 on the VP2 protein of infectious bursal disease virus(IBDV)were synthesized and purified,then were conjugated to carrier protein BSA or OVA respectively.The BSA-conjugated PJ14 and PJ5 were inoculated into the BALB/c mice respectively.The OVA-conjugated PJ14 was used as the antigen to detect the anti-PJ14 antibody in the serum of the immunized mice,and so was the OVA-conjugated PJ5.The results showed that the PJ14 and PJ5 after the conjugation to BSA stimulated the production of the anti-PJ14 and anti-PJ5 antibody respectively,and that the antibodies lasted for twenty-one weeks.It indicated the immunogenicity of the PJ14 and PJ5.

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